FACE Analysis as a Fast and Reliable Methodology to Monitor the Sulfation and Total Amount of Chondroitin Sulfate in Biological Samples of Clinical Importance
| Autor: | Panagiotis Gartaganis, Alberto Passi, Athanasia P. Asimakopoulou, Antonio Rossi, Nikos Afratis, Vassiliki Zafeiropoulou, Nikos K. Karamanos, Luca Monti, Evgenia Karousou |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Electrophoresis
high performance liquid chromatography Capillary electrophoresis Chondroitin sulfate Fluorophore-assisted carbohydrate electrophoresis Glycosaminoglycans High performance liquid chromatography Hyaluronan capillary electrophoresis Pharmaceutical Science Diagnostic tools Article Analytical Chemistry lcsh:QD241-441 hyaluronan chemistry.chemical_compound Sulfation Blood serum lcsh:Organic chemistry Drug Discovery Sample preparation Physical and Theoretical Chemistry Chromatography High Pressure Liquid chondroitin sulfate Chromatography Chemistry Chondroitin Sulfates Organic Chemistry Electrophoresis Capillary Face analysis glycosaminoglycans fluorophore-assisted carbohydrate electrophoresis Biochemistry Chemistry (miscellaneous) Molecular Medicine |
| Zdroj: | Molecules; Volume 19; Issue 6; Pages: 7959-7980 Molecules Molecules, Vol 19, Iss 6, Pp 7959-7980 (2014) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules19067959 |
| Popis: | Glycosaminoglycans (GAGs) due to their hydrophilic character and high anionic charge densities play important roles in various (patho)physiological processes. The identification and quantification of GAGs in biological samples and tissues could be useful prognostic and diagnostic tools in pathological conditions. Despite the noteworthy progress in the development of sensitive and accurate methodologies for the determination of GAGs, there is a significant lack in methodologies regarding sample preparation and reliable fast analysis methods enabling the simultaneous analysis of several biological samples. In this report, developed protocols for the isolation of GAGs in biological samples were applied to analyze various sulfated chondroitin sulfate- and hyaluronan-derived disaccharides using fluorophore-assisted carbohydrate electrophoresis (FACE). Applications to biologic samples of clinical importance include blood serum, lens capsule tissue and urine. The sample preparation protocol followed by FACE analysis allows quantification with an optimal linearity over the concentration range 1.0–220.0 µg/mL, affording a limit of quantitation of 50 ng of disaccharides. Validation of FACE results was performed by capillary electrophoresis and high performance liquid chromatography techniques. |
| Databáze: | OpenAIRE |
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