Inducible nitric oxide synthase gene expression and enzyme activity correlate with disease activity in murine experimental autoimmune encephalomyelitis
| Autor: | Pamela S. Wyatt, Charles P. Rodi, Manuel San, Pamela T. Manning, Richard M. Keeling, Anne H. Cross, Salil Goorha, Thomas P. Misko |
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| Rok vydání: | 1996 |
| Předmět: |
Encephalomyelitis
Autoimmune Experimental Encephalomyelitis Immunology Central nervous system Gene Expression Mice Inbred Strains Polymerase Chain Reaction Nitric oxide Mice chemistry.chemical_compound Gene expression medicine Citrulline Animals Immunology and Allergy RNA Messenger biology Experimental autoimmune encephalomyelitis medicine.disease Adoptive Transfer Molecular biology Reverse transcriptase Nitric oxide synthase medicine.anatomical_structure Spinal Cord Neurology chemistry Enzyme Induction biology.protein Female Neurology (clinical) Nitric Oxide Synthase |
| Zdroj: | Journal of Neuroimmunology. 71:145-153 |
| ISSN: | 0165-5728 |
| Popis: | Messenger RNA encoding inducible NO synthase (iNOS) was measured by competitive reverse transcriptase polymerase chain reaction (cRT-PCR) and ribonuclease protection assays in spinal cords from mice at varying stages of experimental allergic encephalomyelitis (EAE) and from control mice. iNOS mRNA was increased in spinal cords from mice with acute EAE. cRT-PCR assays revealed a 10-20-fold increase in iNOS mRNA in spinal cords during acute EAE compared with the level observed in normal mouse spinal cords. Functional iNOS activity, as assessed by assay of calcium-independent citrulline production, was also significantly increased in spinal cords from mice with acute EAE in comparison to normal controls. The correlation of functional iNOS expression with active disease in EAE in consistent with a pathogenic role for excess NO in this model of cell-mediated central nervous system autoimmunity. |
| Databáze: | OpenAIRE |
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