Simple, rapid and sensitive method for the determination of indomethacin in plasma by high-performance liquid chromatography with ultraviolet detection
| Autor: | Juichi Sato, Yuichi Niida, Takasuke Amizuka, Keiji Ito, Masao Umetsu |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Detection limit
Chromatography medicine.diagnostic_test Mefenamic acid Chemistry Indomethacin Infant Newborn General Chemistry High-performance liquid chromatography Sensitivity and Specificity Indometacin Therapeutic drug monitoring Spectrophotometry Tocolytic medicine Humans Spectrophotometry Ultraviolet Drug Monitoring Quantitative analysis (chemistry) Ductus Arteriosus Patent Chromatography High Pressure Liquid Infant Premature medicine.drug |
| Zdroj: | Journal of chromatography. B, Biomedical sciences and applications. 692(1) |
| ISSN: | 1387-2273 |
| Popis: | A micro method for determination of indomethacin in plasma was developed. Following deproteinization of plasma with acetonitrile containing internal standard (mefenamic acid), the separation of indomethacin and internal standard was achieved by high-performance liquid chromatography using a 7 microm LiChrosorb-RP18 column (250x4 mm I.D.) at 50 degrees C. The mobile phase was 6 mM phosphoric acid-acetonitrile (50:50). The flow-rate was kept at 2.0 ml/min and the column effluent was monitored at 205 nm. The coefficients of variation of the method estimated at 0.2 and 1.0 microg/ml were 4.2 and 2.3%, and the detection limit of the drug was about 0.05 microg/ml (S/N=5). The method requires minimum pretreatment of the plasma with a small sample volume (25 microl), and is very suitable for therapeutic drug monitoring of indomethacin in premature infants with symptomatic patent ductus arteriosus. |
| Databáze: | OpenAIRE |
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