Targeting both rs12979860 and rs8099917 Polymorphisms with a Single-Tube High-Resolution Melting Assay for IL28B Genotyping
| Autor: | Evelyn Siew-Chuan Koay, Kok-Siong Poon, Cui Wen Chua, Lily Chiu, Sherry Sze Yee Ho, Julian W. Tang |
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| Rok vydání: | 2012 |
| Předmět: |
Microbiology (medical)
Genotype Hepatitis C virus Hepacivirus Biology Nucleic Acid Denaturation medicine.disease_cause Compound heterozygosity Polymorphism Single Nucleotide High Resolution Melt chemistry.chemical_compound Virology medicine Humans Transition Temperature Gene Genotyping Singapore Clinical Laboratory Techniques Interleukins Haplotype Hepatitis C Molecular biology Haplotypes Molecular Diagnostic Techniques chemistry Interferons DNA |
| Zdroj: | Journal of Clinical Microbiology. 50:3353-3355 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.01718-12 |
| Popis: | A rapid, duplex, high-resolution melting interleukin-28B gene ( IL28B ) genotyping assay, targeting both rs12979860 and rs8099917 polymorphisms, was developed and validated using 30 DNA samples from healthy volunteers. A linkage study on 300 healthy Singaporeans showed variable haplotypes. When the assay was applied to plasma DNA from 50 hepatitis C virus genotype-1 (HCV-1)-infected patients, five compound heterozygous types were detected. |
| Databáze: | OpenAIRE |
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