High yield expression of Lipase A from Candida antarctica in the methylotrophic yeast Pichia pastoris and its purification and characterisation
| Autor: | Sven M. Richter, Carl-Erik Hansen, Monika Rusnak, Rachid Bel Rhlid, Rolf D. Schmid, Jens Nieveler, Jan Pfeffer |
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| Rok vydání: | 2006 |
| Předmět: |
Triacylglycerol lipase
Applied Microbiology and Biotechnology Gene Expression Regulation Enzymologic Pichia Pichia pastoris Gene Expression Regulation Fungal Enzyme Stability Lipase Candida chemistry.chemical_classification biology Temperature General Medicine Hydrogen-Ion Concentration biology.organism_classification Yeast Kinetics Enzyme Biochemistry chemistry Yield (chemistry) Fermentation biology.protein Candida antarctica Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 72:931-938 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s00253-006-0400-z |
| Popis: | The current investigation focuses on shedding further light on the characteristics of lipase A from Candida antarctica (CalA), which has attracted growing attention in its suitability for industrial applications. CalA was functionally expressed in the methylotrophic yeast Pichia pastoris, purified and characterised. A classical fed-batch process and a semi-continuous process were developed and tested with regard to their yield capacity. Lipase concentrations of 0.88 and 0.55 g l(-1) were obtained using the fed-batch and semi-continuous processes, respectively. The semi-continuous process reaches a total activity of 10,233,000 U and so surpasses the fed-batch process reaching 7,530,000 U. The purified enzyme showed highest activity between 50 and 70 degrees C at pH 7.0 and a preference for short-chain triglycerides (C4-C8). Significantly reduced activity was observed in the presence of hydrophilic esters. |
| Databáze: | OpenAIRE |
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