An evaluation of the applicability of microarrays for monitoring toxic algae in Irish coastal waters
| Autor: | Robin Raine, Nicolas Touzet, Gerard T.A. Fleming, Gary R. McCoy |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
0106 biological sciences
Microarray Prymnesium Health Toxicology and Mutagenesis 01 natural sciences 03 medical and health sciences Algae Environmental monitoring Phytoplankton Microalgae Environmental Chemistry Ecotoxicology Seawater 14. Life underwater Toxic algae Oligonucleotide Array Sequence Analysis 030304 developmental biology 0303 health sciences biology Ecology 010604 marine biology & hydrobiology General Medicine biology.organism_classification Pollution Fishery Marine Toxins DNA microarray Ireland Water Pollutants Chemical Environmental Monitoring |
| Zdroj: | Environmental Science and Pollution Research. 20:6751-6764 |
| ISSN: | 1614-7499 0944-1344 |
| Popis: | The applicability of microarrays to monitor harmful algae across a broad range of ecological niches and toxic species responsible for harmful algal events has been one of the key tasks in the EU Seventh Framework Programme (FP7)-funded Microarrays for the Detection of Toxic Algae project. The technique has a strong potential for improving speed and accuracy of the identification of harmful algae and their toxins to assist monitoring programmes. Water samples were collected from a number of coastal sites around Ireland, including several that are used in the Irish National Phytoplankton and Biotoxin Monitoring Programme. Ribosomal RNA was extracted from filtered field samples, labelled with a fluorescent dye, and hybridised to probes spotted in a microarray format on a glass slide. The fluorescent signal intensity of the hybridisation to120 probes on the chip was analysed and compared with actual field counts. There was a general agreement between cell counts and microarray signal. Results are presented for field samples taken from a range of stations along the Irish coastline known for harmful algal events during the first field trial (July 2009-April 2010). |
| Databáze: | OpenAIRE |
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