Rapid group-, serotype-, and vaccine strain-specific identification of poliovirus isolates by real-time reverse transcription-PCR using degenerate primers and probes containing deoxyinosine residues

Autor:	Allan Nix, Chen-Fu Yang, Olen M. Kew, Su-Ju Yang, Ray Campagnoli, Karen Ching, Annelet Vincent, Lina De, Jane Iber, David R. Kilpatrick, Mark Mandelbaum
Rok vydání:	2009
Předmět:	Microbiology (medical) Serotype Biology medicine.disease_cause urologic and male genital diseases complex mixtures Sensitivity and Specificity Virus Microbiology law.invention law Virology medicine Humans Polymerase chain reaction DNA Primers Reverse Transcriptase Polymerase Chain Reaction Poliovirus Reverse transcriptase female genital diseases and pregnancy complications Reverse transcription polymerase chain reaction Enterovirus RNA Viral Oligonucleotide Probes Specific identification
Zdroj:	Journal of clinical microbiology. 47(6)
ISSN:	1098-660X
Popis:	We have adapted our previously described poliovirus diagnostic reverse transcription-PCR (RT-PCR) assays to a real-time RT-PCR (rRT-PCR) format. Our highly specific assays and rRT-PCR reagents are designed for use in the WHO Global Polio Laboratory Network for rapid and large-scale identification of poliovirus field isolates.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e6876054d81cc6ce305b820a3f4d6e3 https://pubmed.ncbi.nlm.nih.gov/19386844 Zobrazit plný text záznamu