Apoptosis and Cellular Activation in the Pathogenesis of Acute Vascular Rejection
| Autor: | Josie Williams, Gregory J. Brunn, Matilda Bustos, Gregory J. Gores, Zoie E. Holzknecht, Karisha L. Kuypers, Jeffrey L. Platt, Timothy B. Plummer |
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| Rok vydání: | 2002 |
| Předmět: |
Graft Rejection
Programmed cell death Pathology medicine.medical_specialty Endothelium Swine Physiology Guinea Pigs Transplantation Heterologous Gene Expression Apoptosis CD59 Antigens Biology Pathogenesis Necrosis In Situ Nick-End Labeling medicine Animals Humans Fibrin TUNEL assay Myocardium Rats Transplantation Endothelial stem cell Disease Models Animal medicine.anatomical_structure Rats Inbred Lew Acute Disease Antibody Formation Disease Progression Blood Vessels Heart Transplantation Endothelium Vascular Cardiology and Cardiovascular Medicine Papio Blood vessel |
| Zdroj: | Circulation Research. 91:1135-1141 |
| ISSN: | 1524-4571 0009-7330 |
| DOI: | 10.1161/01.res.0000046236.20251.fa |
| Popis: | Acute vascular or humoral rejection, a vexing outcome of organ transplantation, has been attributed by some to activation and by others to apoptosis of endothelial cells in the graft. We asked which of these processes causes acute vascular rejection by tracing the processes during the development of acute vascular rejection in porcine cardiac xenografts performed in baboons. Apoptosis, assayed by terminal deoxynucleotidyl transferase–mediated dUTP nick end-labeling (TUNEL), expression of activated caspase-3, and proapoptotic genes Bax and Bcl-xL, was not detected until acute vascular rejection was well advanced, and even then, apoptosis was largely confined to myocytes. Activation of the endothelium, as evidenced by expansion of rough endoplasmic reticulum and increased ribosomal antigen and phospho-p70 S6 kinase, occurred early in the course of acute vascular rejection and progressed through the disease process. These findings suggest that acute vascular rejection is caused by an active metabolic process and not by apoptosis in the endothelium. |
| Databáze: | OpenAIRE |
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