The Antifungal Mechanism of Isoxanthohumol from Humulus lupulus Linn
| Autor: | Wu Zhengrong, Yin-Fang Yan, Du Shasha, Wu Tianlin, Cheng-Jie Yang, Zhi-Jun Zhang, Zhao Wenbin, Yong-Mei Hu, Ying-Qian Liu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Humulus lupulus
Antifungal Agents QH301-705.5 Xanthones Flavonoid isoxanthohumol Catalysis Article Inorganic Chemistry chemistry.chemical_compound Botrytis cinerea Adenosine Triphosphate In vivo Physical and Theoretical Chemistry Biology (General) Humulus Molecular Biology QD1-999 Spectroscopy EC50 chemistry.chemical_classification biology Traditional medicine Isoxanthohumol Chemistry Organic Chemistry fungi antifungal activity Humulus lupulus Linn food and beverages General Medicine Metabolism biology.organism_classification Computer Science Applications Fungicide Botrytis Lipid Peroxidation |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 22, Iss 10853, p 10853 (2021) Volume 22 Issue 19 |
| ISSN: | 1422-0067 |
| Popis: | Humulus lupulus Linn. is a traditional medicinal and edible plant with several biological properties. The aims of this work were: (1) to evaluate the in vitro antifungal activity of H. lupulus ethanolic extract (2) to study the in vitro and in vivo antifungal activity of isoxanthohumol, an isoprene flavonoid from H. lupulus, against Botrytis cinerea and (3) to explore the antifungal mechanism of isoxanthohumol on B. cinerea. The present data revealed that the ethanolic extract of H. lupulus exhibited moderate antifungal activity against the five tested phytopathogenic fungi in vitro, and isoxanthohumol showed highly significant antifungal activity against B. cinerea, with an EC50 value of 4.32 µg/mL. Meanwhile, it exhibited moderate to excellent protective and curative efficacies in vivo. The results of morphologic observation, RNA-seq, and physiological indicators revealed that the antifungal mechanism of isoxanthohumol is mainly related to metabolism it affected the carbohydrate metabolic process, destroyed the tricarboxylic acid (TCA) cycle, and hindered the generation of ATP by inhibiting respiration. Further studies indicated that isoxanthohumol caused membrane lipid peroxidation, thus accelerating the death of B. cinerea. This study demonstrates that isoxanthohumol can be used as a potential botanical fungicide for the management of phytopathogenic fungi. |
| Databáze: | OpenAIRE |
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