Novel probiotic yeast from Miso promotes regulatory dendritic cell IL-10 production and attenuates DSS-induced colitis in mice
| Autor: | Masaaki Higashiyama, Kengo Tomita, Kazuki Horiuchi, Naoki Shibuya, Akinori Mizoguchi, Chikako Watanabe, Yoshinori Hanawa, Nao Sugihara, Kenichi Inaba, Chie Kurihara, Yoshikiyo Okada, Soichiro Miura, Yoshikazu Tsuzuki, Akinori Wada, Suguru Itoh, Rina Tanemoto, Shunsuke Komoto, Shin Nishii, Ryota Hokari |
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| Rok vydání: | 2020 |
| Předmět: |
Adoptive cell transfer
Microbiology law.invention Probiotic Mice Japan law medicine Mesenteric lymph nodes Animals Colitis Chemistry Probiotics Gastroenterology Interleukin Soy Foods hemic and immune systems Dendritic cell Dendritic Cells medicine.disease Interleukin-10 Mice Inbred C57BL TLR2 Interleukin 10 Disease Models Animal medicine.anatomical_structure |
| Zdroj: | Journal of gastroenterology. 56(9) |
| ISSN: | 1435-5922 |
| Popis: | Yeasts are a type of fungi thought to have probiotic functions. In this study, we isolated a novel probiotic yeast (Zygosaccharomyces sapae strain I-6) from Miso (a traditional Japanese fermented food). We examined its effects on phenotypic changes in intestinal dendritic cells (DCs), and evaluated its anti-inflammatory effects in dextran sulfate sodium (DSS)-induced colitis. A single colony was selected from homogenized Miso, based on its ability to produce interleukin (IL)-10 in CD11c+ bone marrow DCs (BMDCs) in vitro. The anti-inflammatory effects of strain I-6 on CD11c+ BMDCs and CD11c+ CD103+ DCs were analyzed in mouse mesenteric lymph nodes in vitro and in a DSS mouse model. The IL-10 concentrations in the co-culture BMDC supernatants treated with I-6 were dramatically higher than in those treated with Saccharomyces cerevisiae (Sc). IL-10 production is mediated by both TLR2 and Dectin-1. β-Glucan extracted from I-6 also induced higher levels of IL-10 production in BMDCs than β-glucan from Sc. The number of mesenteric lymph node CD11c+ CD103+ DCs was significantly increased by I-6 administration, compared with Sc administration. Strain I-6 showed strong anti-inflammatory effects on DSS-induced colitis compared to Sc. Moreover, the adoptive transfer of I-6-treated BMDCs showed anti-inflammatory effects on DSS-induced colitis in mice without oral administration of I-6 cells. Strain I-6 induced phenotypic changes in intestinal CD11c+ DCs characterized by high IL-10 production and exerted strong anti-inflammatory effects on DSS-induced colitis. Traditional Japanese fermented foods may be a valuable source of probiotic yeasts for effective IBD therapy and treatment. |
| Databáze: | OpenAIRE |
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