The preparation of chitosan membrane improved with nanoparticles based on unsaturated fatty acid for using in cancer-related infections
Autor: | Baki Hazer, Abdullah Kizaloglu, Emir Baki Denkbaş, Ebru Kilicay, Zeynep Karahaliloğlu |
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Přispěvatelé: | Sabire Yazıcı Fen Edebiyat Fakültesi, Kapadokya Üniversitesi, Uygulamalı Bilimler Yüksekokulu, Uçak Gövde ve Motor Bakımı Bölümü |
Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
Polymers and Plastics
Carrier system Cancer-associated Infections Nanoparticle Bioengineering 02 engineering and technology 010402 general chemistry 01 natural sciences Biomaterials Chitosan chemistry.chemical_compound Materials Chemistry Caffeic acid medicine Unsaturated fatty acid Chemistry technology industry and agriculture Cancer 021001 nanoscience & nanotechnology medicine.disease 0104 chemical sciences Oleic acid Chitosan membrane Caffeic Acid 0210 nano-technology Nuclear chemistry Oleic Acid |
Popis: | Karahaliloǧlu, Zeynep ( Aksaray, Yazar ) This study includes the design of a chitosan membrane decorated with unsaturated fatty acid–based carrier system for cancer treatment and antibacterial application. For this, polystyrene-graft-polyoleic acid-graft-polyethylene glycol was prepared by free radical polymerization and characterized. Nanoparticles and caffeic acid–loaded nanoparticles were prepared by solvent evaporation technique and optimized. The short-term stability of nanoparticles was investigated at 4°C. Drug encapsulation and loading efficiency were evaluated. The chitosan membrane and caffeic acid–loaded nanoparticles embedded into chitosan membrane were fabricated. The caffeic acid loaded nanoparticles embedded into chitosan membrane showed controlled release. The mechanical properties of all samples were investigated. The caffeic acid–loaded nanoparticles embedded into chitosan membranes indicated excellent antibacterial properties against the Escherichia coli and Staphylococcus aureus. The anticancer activity of all the samples was evaluated against SaOS-2 human primary osteogenic sarcoma and MC3T3-E1 pre-osteoblast cell lines by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay, the flow cytometry and double staining methods. As a result, the designed carrier system showed great potential to cancer-associated infections treatment in bone cancer cases. |
Databáze: | OpenAIRE |
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