SARS-CoV-2 Multi-Antigen Serology Assay
| Autor: | Stephanie R. Hyslop, Leanne J. Robinson, Ivo Mueller, Catherine Chen, Nicholas Kiernan-Walker, Deborah A Williamson, Fiona Angrisano, Shazia Ruybal-Pesántez, Ramin Mazhari, Emily M. Eriksson, Caitlin Bourke, Rhea J. Longley |
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| Rok vydání: | 2021 |
| Předmět: |
Antibody measurement
Coronavirus disease 2019 (COVID-19) biology SARS-CoV-2 QH301-705.5 business.industry Transmission (medicine) Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Serological assay COVID-19 serology Biochemistry Genetics and Molecular Biology (miscellaneous) Virology Serology Antigen Structural Biology Protocol Luminex biology.protein Medicine Biology (General) Antibody business Biotechnology |
| Zdroj: | Methods and Protocols, Vol 4, Iss 72, p 72 (2021) Methods and Protocols |
| ISSN: | 2409-9279 |
| DOI: | 10.3390/mps4040072 |
| Popis: | Serology tests are extremely useful for assessing whether a person has been infected with a pathogen. Since the onset of the COVID-19 pandemic, measurement of anti-SARS-CoV-2-specific antibodies has been considered an essential tool in identifying seropositive individuals and thereby understanding the extent of transmission in communities. The Luminex system is a bead-based technology that has the capacity to assess multiple antigens simultaneously using very low sample volumes and is ideal for high-throughput studies. We have adapted this technology to develop a COVID-19 multi-antigen serological assay. This protocol described here carefully outlines recommended steps to optimize and establish this method for COVID-19-specific antibody measurement in plasma and in saliva. However, the protocol can easily be customized and thus the assay is broadly applicable to measure antibodies to other pathogens. |
| Databáze: | OpenAIRE |
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