Effect of adjuvants in the isotypes and bactericidal activity of antibodies against the transferrin-binding proteins of Neisseria meningitidis
| Autor: | Carlos M. Ferreirós, José Antonio Caride Gómez, E. Hernández, M.T. Criado |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Blood Bactericidal Activity
medicine.medical_treatment Cross Reactions Neisseria meningitidis Biology medicine.disease_cause Microbiology Mice Immune system Adjuvants Immunologic Bacterial Proteins Antigen Iron-Binding Proteins medicine Animals Mice Inbred BALB C General Veterinary General Immunology and Microbiology Immunogenicity Public Health Environmental and Occupational Health Transferrin-Binding Proteins Antibodies Bacterial Isotype Immunoglobulin Isotypes Infectious Diseases Humoral immunity Immunology biology.protein Molecular Medicine Antibody Carrier Proteins Adjuvant |
| Zdroj: | Vaccine. 16:1633-1639 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/s0264-410x(98)00062-0 |
| Popis: | Twenty-eight Neisseria meningitidis strains of different serogroups, serotypes, and TbpB isotypes were used to test the effect of five adjuvant formulations on the immune response to the meningococcal transferrin-binding proteins (Tbps) in mice. Levels of anti-Tbps antibodies were relatively low when purified TbpA-TbpB complexes were used for immunization, those obtained with the RAS adjuvant being the highest, and the isotype distribution reveals a prevalence of the non-bactericidal IgG1. Specific anti-Tbps antibody levels were five to 125 times higher immunizing with whole outer membrane vesicles, with bactericidal isotypes prevailing, which suggests that presentation of these antigens in their natural conformation is crucial to elicit a good response. Nevertheless, bactericidal activity did not correlate with these characteristics, confirming that it must be also influenced by other factors, and direct evaluation of the killing ability is necessary to draw conclusions about the efficacy of antigens or adjuvants in vaccine design. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect