Microscopic localization of PEG-liposomes in a rat model of focal infection

Autor: Peter Laverman, Frans H.M. Corstens, E.T.M. Dams, Gert Storm, Otto C. Boerman, Wim J.G. Oyen, Theo Hafmans, H.J.E. Croes
Rok vydání: 2001
Předmět:
Male
Pathology
medicine.medical_specialty
Phagocytosis
Pharmaceutical Science
Inflammation
Spleen
Vascular permeability
Biology
Role of fatty acid-binding proteins
proteoglycans and ion transport in differentiation and pathology
Polyethylene Glycols
De rol van cytokinen in de pathofysiologie van koortsende ziekten en in de afweer tegen infecties
De rol van vetzuurbindende eiwitten
proteoglycanen en iontransport bij differentiatie en pathologie
medicine
Animals
Tissue Distribution
Rats
Wistar
Drug Carriers
Liposome
Development of radiopharmaceuticals for diagnosis and therapy of pathological processes
The role of cytokines in the pathophysiology of febrile illnesses and in host defense against infections
Staphylococcal Infections
Abscess
Extravasation
Rats
Ontwikkeling van radiofarmaca ten behoeve van diagnose en behandeling van ziekteprocessen
Microscopy
Electron
medicine.anatomical_structure
Liver
Colloidal gold
Liposomes
Red pulp
medicine.symptom
Zdroj: Journal of Controlled Release, 75, 3, pp. 347--55
Journal of Controlled Release, 75, 347--55
ResearcherID
ISSN: 0168-3659
Popis: Item does not contain fulltext In the present study the microscopic localization of polyethylene glycol (PEG) liposomes in infected tissues was studied with both light microscopy (LM) and transmission electron microscopy (TEM) in rats with focal intramuscular Staphylococcus aureus infection. PEG-liposomes containing colloidal gold were prepared and injected intravenously in rats with focal S. aureus infection and tissues were dissected at 24 h post injection. Sections were cut and liposomes were visualized for microscopic evaluation using silver enhancement. Uptake of PEG-liposomes was visualized by both scintigraphy and LM in the abscess, liver and spleen. In the infected area, the liposomes were mainly found in the vicinity of blood vessels. TEM showed that the liposomes were localized in the macrophages and to a lesser extent in endothelial cells in the infectious tissue. In the liver, the liposomes appeared mainly localized in Kupffer cells. In the spleen, uptake was only seen in cells of the red pulp and in cells around the central arteries. Our microscopic observations indicate that uptake and retention of PEG-liposomes in the infectious focus is a result of enhanced extravasation due to increased vascular permeability and subsequent phagocytosis of PEG-liposomes by macrophages in the infected tissue.
Databáze: OpenAIRE
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