Phase optimized liquid chromatography as an instrument for steroid analysis

Autor:	Klaus Albert, Manuela Gradl, Benjamin Dietrich, Karsten Holtin, Maximilian Kuehnle, Jens Rehbein, Helen Yeman
Rok vydání:	2008
Předmět:	Electrospray Chromatography Chemistry medicine.medical_treatment Analytical chemistry Filtration and Separation Mass spectrometry High-performance liquid chromatography Analytical Chemistry Steroid Stationary phase Phase (matter) medicine Theoretical plate Selectivity
Zdroj:	Journal of separation science. 31(10)
ISSN:	1615-9314
Popis:	A new method using phase optimized LC (POPLC) for the analysis of steroids is described. The retention factors and the theoretical plate numbers of different steroids were determined for four different stationary phases. Based on these values, an optimal stationary phase composition and the resultant chromatogram have been calculated by an optimization software and compared to the measured data. It is demonstrated that these predicted data show only little differences to the experimental results. Furthermore, it is shown that the overall selectivity of the optimal stationary phase composition is much better than the selectivity of any individual stationary phase.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e5f11dc012c51c79a20ce6fea99c221 https://pubmed.ncbi.nlm.nih.gov/18461641 Zobrazit plný text záznamu Plný text