Induced cell proliferation: The effect of isoproterenol
| Autor: | T. Barka |
|---|---|
| Rok vydání: | 1965 |
| Předmět: |
medicine.medical_specialty
Cell division Submandibular Gland Biology Tritium Muscle hypertrophy chemistry.chemical_compound Internal medicine medicine Cell Proliferation Pharmacology Hyperplasia Cell growth Research Isoproterenol DNA Hypertrophy Organ Size Cell Biology Metabolism medicine.disease Submandibular gland Rats Kinetics Endocrinology medicine.anatomical_structure chemistry Autoradiography Specific activity Thymidine Cell Division |
| Zdroj: | Experimental Cell Research. 37:662-679 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/0014-4827(65)90214-4 |
| Popis: | Isoproterenol (IPR) in pharmacologic doses produces enlargement of the salivary glands in rat and mouse. Alterations in weight, DNA-content and incorporation of 3H-thymidine in the submaxillary gland of rat were studied using autoradiographic and chemical techniques during a four-week course of IPR treatment. Within 2 days 80 to 100 mg of IPR markedly stimulated the incorporation of 3H-thymidine into the nuclei of acinar cells of the submaxillary gland and into the nuclei of hepatocytes. Continuous administration of the drug produced lesser peaks of labeling by the 7th and 21st day. The percentage of labeled interstitial and duct cells was the highest after treatment for seven days. The weight of the submaxillary gland significantly increased after the administration of IPR for 4 days and reached its maximum 3.5 times normal, after 2 weeks. The amount of DNA per gland was also significantly higher after 4 days of IPR treatment increasing further up to 14 days. The incorporation of 3H-thymidine into DNA per gland was severalfold above control level from the second day on with a 1255 per cent peak after injections of IPR for 4 days. The specific activity of DNA rose to 8 times the control after only 2 days of IPR treatment. These alterations quickly regressed after withdrawal of the drug. Studies on the turnover rate of DNA indicated reutilization of DNA or its catabolites by the cells of submaxillary gland during IPR treatment. Dichloroisoproterenol caused no alteration of the weight, DNA-content and incorporation of thymidine in the submaxillary gland and it partly prevented the effect of IPR. |
| Databáze: | OpenAIRE |
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