The role of the nAChR subunits α5, β2, and β4 on synaptic transmission in the mouse superior cervical ganglion

Autor:	Petra Scholze, Rudolf Karch, Xenia Simeone, Anna Ciuraszkiewicz, Avi Orr-Urtreger, Sigismund Huck, Rosa Lemmens-Gruber
Rok vydání:	2019
Předmět:	Superior cervical ganglion Physiology Compound action potential Ganglionic Blockers Stimulation Nerve Tissue Proteins Nicotinic Antagonists Superior Cervical Ganglion 030204 cardiovascular system & hematology Neurotransmission Receptors Nicotinic Synaptic Transmission Hexamethonium lcsh:Physiology Signalling Pathways 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Physiology (medical) Animals Receptor Cells Cultured Acetylcholine receptor Original Research Ganglionic blocking agent Mice Knockout Neurons lcsh:QP1-981 EPSP Synaptic Potentials nicotinic ACh receptor Molecular biology 3. Good health Mice Inbred C57BL Nicotinic agonist chemistry 030217 neurology & neurosurgery knockout mice Neuroscience
Zdroj:	Physiological Reports Physiological Reports, Vol 7, Iss 6, Pp n/a-n/a (2019)
ISSN:	2051-817X
Popis:	Our previous immunoprecipitation analysis of nicotinic acetylcholine receptors (nAChRs) in the mouse superior cervical ganglion (SCG) revealed that approximately 55%, 24%, and 21% of receptors are comprised of α3β4, α3β4α5, and α3β4β2 subunits, respectively. Moreover, mice lacking β4 subunits do not express α5‐containing receptors but still express a small number of α3β2 receptors. Here, we investigated how synaptic transmission is affected in the SCG of α5β4‐KO and α5β2‐KO mice. Using an ex vivo SCG preparation, we stimulated the preganglionic cervical sympathetic trunk and measured compound action potentials (CAPs) in the postganglionic internal carotid nerve. We found that CAP amplitude was unaffected in α5β4‐KO and α5β2‐KO ganglia, whereas the stimulation threshold for eliciting CAPs was significantly higher in α5β4‐KO ganglia. Moreover, intracellular recordings in SCG neurons revealed no difference in EPSP amplitude. We also found that the ganglionic blocking agent hexamethonium was the most potent in α5β4‐KO ganglia (IC 50: 22.1 μmol/L), followed by α5β2‐KO (IC 50: 126.7 μmol/L) and WT ganglia (IC 50: 389.2 μmol/L). Based on these data, we estimated an IC 50 of 568.6 μmol/L for a receptor population consisting solely of α3β4α5 receptors; and we estimated that α3β4α5 receptors comprise 72% of nAChRs expressed in the mouse SCG. Similarly, by measuring the effects of hexamethonium on ACh‐induced currents in cultured SCG neurons, we found that α3β4α5 receptors comprise 63% of nAChRs. Thus, in contrast to our results obtained using immunoprecipitation, these data indicate that the majority of receptors at the cell surface of SCG neurons consist of α3β4α5.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e5ad6f1b4154bd97d2c0e08be09474f https://pubmed.ncbi.nlm.nih.gov/30891952 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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