Alterations in platelet function in patients receiving interleukin-6 as cytokine therapy
| Autor: | Leslie Oleksowicz, E G Puszkin, Janice P. Dutcher, Randi Isaacs, Zbigniew Mrowiec |
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| Rok vydání: | 1996 |
| Předmět: |
Blood Platelets
Cancer Research medicine.medical_specialty Epinephrine Platelet Aggregation medicine.medical_treatment Radioimmunoassay Antineoplastic Agents Enzyme-Linked Immunosorbent Assay In Vitro Techniques Platelet Factor 4 chemistry.chemical_compound Bolus (medicine) Internal medicine Neoplasms Medicine Humans Platelet Sodium dodecyl sulfate Interleukin 6 Infusions Intravenous Interleukin 5 Polyacrylamide gel electrophoresis Arachidonic Acid biology business.industry Interleukin-6 Platelet Count General Medicine Adenosine Diphosphate Thromboxane B2 Kinetics Endocrinology Cytokine Oncology chemistry Injections Intravenous biology.protein business Ex vivo |
| Zdroj: | Cancer investigation. 14(4) |
| ISSN: | 0735-7907 |
| Popis: | Platelet function in 12 cancer patients was studied sequentially over 97 hr of interleukin-6 (IL-6) daily bolus or continuous infusion (C.I.) therapy. During this period, enhanced ex vivo agonist-induced platelet maximum aggregation (MA) was paralleled by an increase in plasma levels of TXB2 and PF4 as measured by RIA and ELISA, respectively. Platelet-rich plasma (PRP) specimens from bolus IL-6-treated patients demonstrated an increased incorporation of actin-binding protein and myosin in the cytoskeletal core (triton insoluble residue) as shown by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in comparison to control specimens. Similarly, the integrin glycoprotein IIIa (GPIIIa) was also observed to be retained into the cytoskeleton by immunoblot. A significant decrease in hypotonic shock response (HSR) was observed over 87 hr of treatment in IL-6 C.I. patients, whereas in IL-6 bolus patients, a significant increase in HSR occurred immediately after the bolus, which was followed by a significant decrease in HSR after 23 hr. These results suggest that IL-6 alters platelet function in vivo. |
| Databáze: | OpenAIRE |
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