Surface recruitment but not activation of integrin alpha IIb beta 3 (GPIIb-IIIa) requires a functional actin cytoskeleton
| Autor: | Dmitriy Grigoryev, Pascal J. Goldschmidt-Clermont, John B. Addo, Nauder Faraday, Paul F. Bray |
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| Rok vydání: | 1995 |
| Předmět: |
Blood Platelets
Cytochalasin D Platelet Aggregation Fibrinogen receptor Protein Conformation Arp2/3 complex Platelet Membrane Glycoproteins Biology Cytoplasmic Granules chemistry.chemical_compound Humans Platelet Actin-binding protein Cytoskeleton Fluorescent Dyes Binding Sites Actin remodeling Fibrinogen Actin cytoskeleton Actins Cell biology Adenosine Diphosphate chemistry biology.protein Cardiology and Cardiovascular Medicine Fluorescein-5-isothiocyanate |
| Zdroj: | Arteriosclerosis, thrombosis, and vascular biology. 15(9) |
| ISSN: | 1079-5642 |
| Popis: | Abstract Binding of integrin α IIb β 3 (glycoprotein [GP] IIb-IIIa) to soluble fibrinogen requires that the receptor undergo a conformational change (receptor activation), which occurs rapidly in agonist-stimulated platelets. Agonist stimulation of platelets also results in α IIb β 3 recruitment from intracellular membranes (α-granules and open canalicular system) to the platelet surface. Once activated and accessible, the receptor can engage, a process that corresponds to the binding of the receptor to its soluble fibrinogen ligand, leading to intracellular signaling reactions and centripetal migration of bound receptor molecules. Because these processes occur concurrently with a marked reorganization of the actin cytoskeleton, we investigated the role of actin in fibrinogen receptor activation and surface recruitment. We used a flow cytometric assay to directly quantitate the binding of α IIb β 3 to fluorescently labeled fibrinogen on the platelet surface. Cytochalasin D, which inhibits elongation of actin filaments, was used to prevent the actin response to platelet agonists. Despite its ability to inhibit the actin response and α IIb β 3 binding to the actin cytoskeleton, cytochalasin D did not alter the agonist-induced intramolecular changes resulting in increased affinity of α IIb β 3 for soluble fibrinogen and therefore did not inhibit ADP-induced aggregation. Thus, disruption of the actin network with cytochalasin D had no effect on the dissociation constant of the complex between activated α IIb β 3 and fibrinogen (K d =0.26 to 0.28 μmol/L). However, cytochalasin D suppressed the recruitment of cryptic α IIb β 3 molecules to the platelet surface. While the physiological consequence of exposing additional α IIb β 3 molecules on the surface of platelets is unclear, it is tempting to speculate that this process plays an important role in consolidating intra-arterial platelet thrombi, despite the shear strain generated by the arterial blood flow. |
| Databáze: | OpenAIRE |
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