WT1 and ACE mRNAs of blood extracellular vesicle as biomarkers of diabetic nephropathy
| Autor: | Alireza Abbasi Baharanchi, Ehsan Hashemi, Ardeshir Kakaei, Saba Zeidi, Farideh Razi, Katayoon Forouzanfar, Hojat Dehghanbanadaki, Seyed Mohammad Mohammadi |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
ELMO1 medicine.medical_specialty Diabetic nephropathy Urine Peptidyl-Dipeptidase A General Biochemistry Genetics and Molecular Biology Nephropathy Pathogenesis Extracellular Vesicles 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Albuminuria Humans Diabetic Nephropathies RNA Messenger WT1 Proteins ACE Adaptor Proteins Signal Transducing business.industry Research Biomarker General Medicine Extracellular vesicle medicine.disease WT1 Cross-Sectional Studies 030104 developmental biology Endocrinology Diabetes Mellitus Type 2 Case-Control Studies 030220 oncology & carcinogenesis Medicine Biomarker (medicine) medicine.symptom business Biomarkers |
| Zdroj: | Journal of Translational Medicine Journal of Translational Medicine, Vol 19, Iss 1, Pp 1-9 (2021) |
| ISSN: | 1479-5876 |
| Popis: | Background Diabetic nephropathy (DN) has an increasing global prevalence with excessive health expenditure and burden. Exosomal mRNAs regulate intercellular communications and participate in the pathogenesis of various disorders like DN. This study aimed to assess the expression levels of ACE, ELMO1, and WT1 mRNAs in the blood extracellular vesicles (EVs) of DN patients and diabetic patients without nephropathy (DM group) in comparison to healthy controls and investigate their correlations with the severity of DN. Methods The performed investigation is a cross-sectional study of 256 participants including 103 DN patients, 100 DM patients, and 53 healthy controls. The quantification of WT1, ACE, and ELMO1 mRNAs in the blood EVs were executed using qRT-PCR. The ROC analysis was performed to determine the diagnostic accuracy of mRNAs. Results DN patients had significantly higher expressed WT1 mRNA (1.70-fold change) and lower expressed ACE mRNA (0.55-fold change) in the blood EVs compared to DM patients and controls. ELMO1 mRNA was not expressed in EVs of any groups. A positive correlation between WT1 mRNA level and urine Alb/Cr ratio (r = 0.602, p Conclusions WT1 and ACE mRNAs level in blood EVs were predictors for early diagnosis of DN therefore their quantifications might be used to determine the severity of albuminuria and glomerular injuries. |
| Databáze: | OpenAIRE |
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