Extensive contribution of embryonic stem cells to the development of an evolutionarily divergent host
| Autor: | Weiqiang Li, Frank Fuxiang Mao, Andy Peng Xiang, John A. Waters, Bruce T. Lahn, Xiuming Zhang, Shu-Nong Li, Xinbing Yu, Jae Hyun Lee, Eric J. Vallender, Tammy W. Vallender, Baofeng Ma, Donghyun Park, Tao Wang, Li Zhang |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Cell type Cellular differentiation Green Fluorescent Proteins Transplantation Heterologous Embryonic Development Biology Polymerase Chain Reaction Genome Germline Animals Genetically Modified Mice Species Specificity Pregnancy Genetics Animals Molecular Biology Embryonic Stem Cells Phylogeny Genetics (clinical) DNA Primers Transplantation Chimera Base Sequence Teratoma Cell Differentiation General Medicine biology.organism_classification Biological Evolution Embryonic stem cell Recombinant Proteins Transplantation Blastocyst Germ Cells Organ Specificity Evolutionary biology Apodemus Female Murinae Stem cell |
| Zdroj: | Human Molecular Genetics. 17:27-37 |
| ISSN: | 1460-2083 0964-6906 |
| DOI: | 10.1093/hmg/ddm282 |
| Popis: | The full potential of embryonic stem (ES) cells to generate precise cell lineages and complex tissues can be best realized when they are differentiated in vivo-i.e. in developing blastocysts. Owing to various practical and ethical constraints, however, it is impossible to introduce ES cells of certain species into blastocysts of the same species. One solution is to introduce ES cells into blastocysts of a different species. However, it is not known whether ES cells can contribute extensively to chimerism when placed into blastocysts of a distantly related species. Here, we address this question using two divergent species, Apodemus sylvaticus and Mus musculus, whose genome sequence differs by approximately 18% from each other. Despite this considerable evolutionary distance, injection of Apodemus ES cells into Mus blastocysts led to viable chimeras bearing extensive Apodemus contributions to all major organs, including the germline, with Apodemus contribution reaching approximately 40% in some tissues. Immunostaining showed that Apodemus ES cells have differentiated into a wide range of cell types in the chimeras. Our results thus provide a proof of principle for the feasibility of differentiating ES cells into a wide range of cell types and perhaps even complex tissues by allowing them to develop in vivo in an evolutionarily divergent host-a strategy that may have important applications in research and therapy. Furthermore, our study demonstrates that mammalian evolution can proceed at two starkly contrasting levels: significant divergence in genome and proteome sequence, yet striking conservation in developmental programs. |
| Databáze: | OpenAIRE |
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