Development of a liquid phase radioimmunoassay for the measurement of serum ferritin levels for the detection of Covid-19 in patients

Autor:	Riad, Emil Michael, Emam, Manal Asem, Ebeid, Nahed Hassan, El-bayoumy, Ahmed Sami, Sallam, Khaled Mohamed, Mehany, Nagy Lahzy, Fathy, Shadia Abdel-Hamid
Rok vydání:	2022
Předmět:	Ferritin Health Toxicology and Mutagenesis Radioimmunoassay I125 Public Health Environmental and Occupational Health Pollution Article Analytical Chemistry Nuclear Energy and Engineering Tracer Labelling Radiology Nuclear Medicine and imaging Covid-19 Spectroscopy
Zdroj:	Journal of Radioanalytical and Nuclear Chemistry
ISSN:	1588-2780 0236-5731
Popis:	The aim of this study was the development and analytically validation of a radioimmunoassay system for the measurement of the serum ferritin concentration as one of the laboratory biomarkers for infection by Covid-19. The main components of the system were prepared in our laboratories. The first component ferritin was extracted and purified from human spleen with high purity. The second component was the 125I-labelled ferritin tracer, prepared using Chloramine-T method. Furthermore anti-ferritin antibodies and ferritin standards were provided. The developed system is sensitive, precise, reproducible and.can be translated into a kit formulation suitable for measuring serum ferritin for the detection of Covid-19 in patients at low costs and high efficiency.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e491518fdc74985874cd9882613070d https://doi.org/10.1007/s10967-022-08208-1 Zobrazit plný text záznamu Plný text ve formátu PDF