The actin cytoskeleton is important for rotavirus internalization and RNA genome replication
| Autor: | Oscar Trejo-Cerro, Carlos F. Arias, Nayeli Aguilar-Hernández, Daniela Silva-Ayala, Susana López |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Rotavirus
Cancer Research media_common.quotation_subject viruses macromolecular substances Biology medicine.disease_cause Virus Replication Virus Article Cell Line 03 medical and health sciences Viral life cycle Viral entry Virology medicine Animals Small GTPase Internalization 030304 developmental biology media_common 0303 health sciences 030306 microbiology Rotavirus genome replication Actin cytoskeleton RNA Epithelial Cells Virus Internalization Macaca mulatta Cell biology Infectious Diseases Rotavirus internalization Host-Pathogen Interactions RNA Viral |
| Zdroj: | Virus Research |
| ISSN: | 1872-7492 |
| Popis: | Highlights • Different stages of the rotavirus lifecycle depend on the dynamics of the actin cytoskeleton. • Alpha-actinin, Diaph, and the GTPase Cdc42 are important for virus entry. • The GTPAse Rac1 is required for maximal viral RNA synthesis. Numerous host factors are required for the efficient replication of rotavirus, including the activation and inactivation of several cell signaling pathways. One of the cellular structures that are reorganized during rotavirus infection is the actin cytoskeleton. In this work, we report that the dynamics of the actin microfilaments are important at different stages of the virus life cycle, specifically, during virus internalization and viral RNA synthesis at 6 h post-infection. Our results show that the actin-binding proteins alpha-actinin 4 and Diaph, as well as the Rho-family small GTPase Cdc42 are necessary for an efficient virus entry, while GTPase Rac1 is required for maximal viral RNA synthesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect