Geminin Participates in Differentiation Decisions of Adult Neural Stem Cells Transplanted in the Hemiparkinsonian Mouse Brain
| Autor: | Eleni Skavatsou, A. Mitsacos, Maria-Eleni Lalioti, Christina Kyrousi, Eve Tasiudi, Zoi Lygerou, Stavros Taraviras, Ioanna Taouki, Konstantina Kaplani, Panagiotis Giompres, Elias D. Kouvelas |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Neurogenesis Biology Mice 03 medical and health sciences Neural Stem Cells Animals Oxidopamine Cells Cultured Cell Proliferation Cell growth Dopaminergic Neurons Dopaminergic Geminin Brain Parkinson Disease Cell Biology Hematology Anatomy Neural stem cell Cell biology Mice Inbred C57BL Transplantation Adult Stem Cells Oligodendroglia 030104 developmental biology embryonic structures biology.protein Stem cell Stem Cell Transplantation Developmental Biology Adult stem cell |
| Zdroj: | Stem Cells and Development. 26:1214-1222 |
| ISSN: | 1557-8534 1547-3287 |
| DOI: | 10.1089/scd.2016.0335 |
| Popis: | Neural stem cells have been considered as a source of stem cells that can be used for cell replacement therapies in neurodegenerative diseases, as they can be isolated and expanded in vitro and can be used for autologous grafting. However, due to low percentages of survival and varying patterns of differentiation, strategies that will enhance the efficacy of transplantation are under scrutiny. In this article, we have examined whether alterations in Geminin's expression, a protein that coordinates the balance between self-renewal and differentiation, can improve the properties of stem cells transplanted in 6-OHDA hemiparkinsonian mouse model. Our results indicate that, in the absence of Geminin, grafted cells differentiating into dopaminergic neurons were decreased, while an increased number of oligodendrocytes were detected. The number of proliferating multipotent cells was not modified by the absence of Geminin. These findings encourage research related to the impact of Geminin on transplantations for neurodegenerative disorders, as an important molecule in influencing differentiation decisions of the cells composing the graft. |
| Databáze: | OpenAIRE |
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