An Evaluation of a Simple Polyacrylamide Gel Electrophoresis System for Lipoproteins and its Use for the Characterisation of Hyperlipoproteinaemia
| Autor: | D. G. Cramp, T. R. Tickner, Ying Foo |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
030213 general clinical medicine
Very low-density lipoprotein Lipoproteins Clinical Biochemistry Hyperlipidemias 030209 endocrinology & metabolism 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Nephelometry and Turbidimetry Humans Polyacrylamide gel electrophoresis Triglycerides Electrophoresis Agar Gel Chromatography Triglyceride Blood Proteins General Medicine Blood Protein Electrophoresis Electrophoresis Cholesterol Biochemistry chemistry Evaluation Studies as Topic Low-density lipoprotein Agarose Electrophoresis Polyacrylamide Gel lipids (amino acids peptides and proteins) Nephelometry Lipoprotein |
| Zdroj: | Annals of Clinical Biochemistry: International Journal of Laboratory Medicine. 16:44-46 |
| ISSN: | 1758-1001 0004-5632 |
| DOI: | 10.1177/000456327901600108 |
| Popis: | SUMMARY The use of polyacrylamide gel electrophoresis as a screening procedure for the character isation of hyperlipoproteinaemia is described. Sixty-one samples were investigated and classified by four different methods: (I) chemical profile derived from visual appearance and estimation ot total cholesterol and triglyceride; (2) 'SML' profile by nephelometry; (3) electrophoresis on agarose gel; and (4) electrophoresis on polyacrylamide gel. Polyacrylamide gel electrophoresis showed the closest correlation with the chemical profile when compared with the other two methods. Polyacrylamide gel electrophoresis was found to be a rapid, reliable, and satisfactory procedure for plasma lipo protein phenotyping. In view of the association of lipid disorders and coronary heart disease, lipid and lipoprotein assays have become an increasingly large part of the routine laboratory workload. The measurement of plasma triglyceride and total cholesterol is the most commonly used and practical means of detecting lipid disorder. However, classification of the hyper lipidaemic states (Beaumont et al., 1970) requires further examination. Lipoprotein electrophoresis has been carried out on paper (Fredrickson and Lees, 1965), cellulose acetate (Coifs and Veheyden, 1967; Chin and Blankenhorn, 1968), and agarose gel (Noble, 1968). The major problem in lipoprotein electrophoresis was the clear separation between very low density lipoprotein (VLDL) and low density lipoprotein (LDL) fraction. Polyacrylamide gel (PAG) has been used for lipoprotein electrophoresis (Naito et al., 1973; Muniz, 1977) and was claimed to be superior in separating VLDL and LDL-and of especial use in type 111. The purpose of this study was to evaluate PAG commercially available as the REDI-DISC kit (Ames Company, Division of Miles Laboratories Ltd) for routine investigation of liproprotein patterns. |
| Databáze: | OpenAIRE |
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