Vitreous Cryopreservation of Human Umbilical Vein Endothelial Cells with Low Concentration of Cryoprotective Agents for Vascular Tissue Engineering
| Autor: | Fazil Panhwar, Gang Zhao, Xiaoming He, Yuanyuan Zheng |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell Survival Biomedical Engineering Medicine (miscellaneous) Bioengineering Article Cryopreservation Umbilical vein 03 medical and health sciences chemistry.chemical_compound Cryoprotective Agents 0302 clinical medicine Tissue engineering Freezing Cryoprotective Agent Human Umbilical Vein Endothelial Cells Humans Vitrification Cells Cultured Volume concentration 030219 obstetrics & reproductive medicine Tissue Engineering Chemistry Dimethyl sulfoxide Temperature 030104 developmental biology Immunology Biophysics Ethylene glycol |
| Zdroj: | Tissue Engineering Part C: Methods. 22:964-973 |
| ISSN: | 1937-3392 1937-3384 |
| Popis: | Cryopreservation of human umbilical vein endothelial cells (HUVECs) is important to tissue engineering applications and the study of the role of endothelial cells in cardiovascular and cerebrovascular diseases. The traditional methods for cryopreservation by vitrification (cooling samples to a cryogenic temperature without apparent freezing) using high concentration of cryoprotective agents (CPAs) and slow freezing are suboptimal due to the severe toxicity of high concentration of CPAs and ice formation-induced cryoinjuries, respectively. In this study, we developed a method to cryopreserve HUVECs by vitrification with low concentration of CPAs. This is achieved by optimizing the CPAs and using highly thermally conductive quartz capillary (QC) to contain samples for vitrification. The latter minimizes the thermal mass to create ultra-fast cooling/warming rates. Our data demonstrate that HUVECs can be vitrified in the QC using 1.4 mol/L ethylene glycol and 1.1 mol/L dimethyl sulfoxide with more than 90% viability. Moreover, this method significantly improves the attachment efficiency of the cryopreserved HUVECs. The attached cells post-cryopreservation proliferate similarly to fresh cells. Therefore, this study may provide an effective vitrification technique to bank HUVECs for vascular tissue engineering and other applications. |
| Databáze: | OpenAIRE |
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