Two-channel autofluorescence analysis for oral cancer
| Autor: | Dong Yuan Wu, Pau-Choo Chung, Chih Yang Chen, Ken Chung Chen, Shoko Nioka, Tze Ta Huang, Ming Hsuan Chang, Yi Chun Chen, Wang Ch Chen, Tung Yiu Wong, Jehn Shyun Huang, Teng Yi Huang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Biomedical Engineering Cancer detection Nicotinamide adenine dinucleotide Sensitivity and Specificity 01 natural sciences 010309 optics Biomaterials Lesion Young Adult 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine 0103 physical sciences Biopsy medicine Humans Aged Aged 80 and over Flavin adenine dinucleotide Redox ratio medicine.diagnostic_test business.industry Mouth Mucosa Discriminant Analysis Middle Aged NAD Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials stomatognathic diseases Autofluorescence Spectrometry Fluorescence Special Section on Metabolic Imaging and Spectroscopy: Britton Chance 105th Birthday Commemorative chemistry 030220 oncology & carcinogenesis Flavin-Adenine Dinucleotide Female Mouth Neoplasms medicine.symptom business |
| Zdroj: | Journal of Biomedical Optics. 24:1 |
| ISSN: | 1083-3668 |
| DOI: | 10.1117/1.jbo.24.5.051402 |
| Popis: | We created a two-channel autofluorescence test to detect oral cancer. The wavelengths 375 and 460 nm, with filters of 479 and 525 nm, were designed to excite and detect reduced-form nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) autofluorescence. Patients with oral cancer or with precancerous lesions, and a control group with healthy oral mucosae, were enrolled. The lesion in the autofluorescent image was the region of interest. The average intensity and heterogeneity of the NADH and FAD were calculated. The redox ratio [(NADH)/(NADH + FAD)] was also computed. A quadratic discriminant analysis (QDA) was used to compute boundaries based on sensitivity and specificity. We analyzed 49 oral cancer lesions, 34 precancerous lesions, and 77 healthy oral mucosae. A boundary (sensitivity: 0.974 and specificity: 0.898) between the oral cancer lesions and healthy oral mucosae was validated. Oral cancer and precancerous lesions were also differentiated from healthy oral mucosae (sensitivity: 0.919 and specificity: 0.755). The two-channel autofluorescence detection device and analyses of the intensity and heterogeneity of NADH, and of FAD, and the redox ratio combined with a QDA classifier can differentiate oral cancer and precancerous lesions from healthy oral mucosae. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|