Effect of N-Acetylcysteine on Lipopolysaccharide-Induced Uveitis in Rats
| Autor: | Yoriko Hayasaka, Hu-Shan Cui, Zai-Long Chi, Xue-Yun Zhang, Seiji Hayasaka |
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| Rok vydání: | 2007 |
| Předmět: |
Lipopolysaccharides
Male Lipopolysaccharide Injections Subcutaneous Iris Nitric Oxide Synthase Type II Proinflammatory cytokine Aqueous Humor chemistry.chemical_compound Ciliary body In vivo Escherichia coli medicine Animals RNA Messenger Rats Wistar Dose-Response Relationship Drug biology Interleukin-6 Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha Chemistry Ciliary Body Interleukin General Medicine Intercellular Adhesion Molecule-1 medicine.disease Uveitis Anterior Molecular biology Acetylcysteine Rats Nitric oxide synthase Disease Models Animal Ophthalmology medicine.anatomical_structure biology.protein Tumor necrosis factor alpha E-Selectin Uveitis |
| Zdroj: | Japanese Journal of Ophthalmology. 51:14-20 |
| ISSN: | 1613-2246 0021-5155 |
| DOI: | 10.1007/s10384-006-0382-5 |
| Popis: | In this study we investigated the in vivo effect of N-acetylcysteine (NAC) on lipopolysaccharide (LPS)-induced uveitis in rats. To induce uveitis, LPS (100 µg) was injected into subcutaneous tissue of Wistar rats (170–190 g). NAC was injected intraperitoneally. Intracameral levels of protein, cells, nitrite, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 were determined by spectrophotometry, hemocytometry, and enzyme-linked immunosorbent assay. Expression of TNF-α, IL-6, endothelial leukocyte adhesion molecule-1 (E-selectin), intercellular adhesion molecule-1 (ICAM-1), and inducible nitric oxide synthase (iNOS) mRNA was examined by real-time polymerase chain reaction. LPS injection elevated intracameral protein and cells, and the elevation was inhibited by NAC. LPS injection induced expression of TNF-α, IL-6, E-selectin, and ICAM-1 mRNA in the iris/ciliary body at 3 h, and iNOS mRNA at 6 h. The LPS-induced elevation of the mRNA levels was inhibited by NAC. NAC inhibited LPS-induced intracameral elevation of TNF-α, IL-6, and nitrite. NAC decreased LPS-induced uveitis in vivo by reducing the expression of proinflammatory cytokines and adhesion molecules. Jpn J Ophthalmol 2007;51:14–20 © Japanese Ophthalmological Society 2007 |
| Databáze: | OpenAIRE |
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