Secreted production of an elastin-like polypeptide by Pichia pastoris
| Autor: | Frits A. de Wolf, Gerrit Eggink, Marc W. T. Werten, Roelof Schipperus, Rosalie L. M. Teeuwen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Bio Process Engineering
medicine.disease_cause Applied Microbiology and Biotechnology recombinant proteins Pichia law.invention Pichia pastoris Drug Stability law DNA Fungal Fungal protein biology General Medicine Hydrogen-Ion Concentration Organische Chemie Elastin-like polypeptides Biochemistry AFSG Biobased Products Recombinant DNA Biotechnology purification Inverse transition cycling Molecular Sequence Data Secreted expression in-vitro Heterologous protein expression Fungal Proteins expression medicine Amino Acid Sequence gene Escherichia coli VLAG Base Sequence Methanol Organic Chemistry biology.organism_classification inverse temperature transitions fermentation process In vitro Biotechnological Products and Process Engineering drug-delivery Elastin Yield (chemistry) Protein Biosynthesis escherichia-coli Fermentation protein-based polymer Peptides |
| Zdroj: | Applied Microbiology and Biotechnology Applied Microbiology and Biotechnology, 85(2), 293-301 Applied Microbiology and Biotechnology 85 (2009) 2 |
| ISSN: | 1432-0614 0175-7598 |
| Popis: | Elastin-like polypeptides (ELPs) are biocompatible designer polypeptides with inverse temperature transition behavior in solution. They have a wide variety of possible applications and a potential medical importance. Currently, production of ELPs is done at lab scale in Escherichia coli shake flask cultures. With a view to future large scale production, we demonstrate secreted production of ELPs in methanol-induced fed-batch cultures of Pichia pastoris and purification directly from the culture medium. The production of ELPs by P. pastoris proved to be pH dependent within the experimental pH range of pH 3 to 7, as an increasing yield was found in cultures grown at higher pH. Because ELP produced at pH 7 was partly degraded, a pH optimum for production of ELP was found at pH 6 with a yield of 255 mg of purified intact ELP per liter of cell-free medium. |
| Databáze: | OpenAIRE |
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