A Systematic Comparative Study of the Toxicity of Semiconductor and Graphitic Carbon-Based Quantum Dots Using In Vitro Cell Models
| Autor: | Maria Carmen Navarro-Ruiz, Miguel Valcárcel, María M. Malagón, Angelina Cayuela, Maria Laura Soriano, Rocío Guzmán-Ruiz |
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| Přispěvatelé: | [Navarro-Ruiz,MC, Guzmán-Ruiz,R, Malagón,MM] Department of Cell Biology, Physiology, and Immunology, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), University of Córdoba, Reina Sofia University Hospital, Córdoba, Spain. [Navarro-Ruiz,MC, Malagón,MM] CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), Instituto de Salud Carlos III, Madrid, Spain. [Cayuela,A, Soriano,ML, Valcárcel,M] Department of Analytical Chemistry, Institute of Fine Chemistry and Nanochemistry, Marie Curie Building, Campus Universitario de Rabanales, University of Córdoba, Córdoba, Spain. [Soriano,ML] Regional Institute for Applied Chemistry Research (IRICA), Ciudad Real, Spain., We acknowledge support from the Spanish Ministry of Science and Innovation for funding the project NANOMIN (CTQ2014-52939-R), Plan Propio de Investigación de la Universidad de Córdoba, MINECO/FEDER (BFU2013-44229-R, BFU2016-76711-R, BFU2017-90578-REDT to M.M.M.), Consejería de Salud y Bienestar Social/J. Andalucía/FEDER (PI-0200/2013 to M.M.M., PI-0159-2016 to G.-R.R.), Instituto de Salud Carlos III (ISCIII) and Fondos FEDER (PIE14/00005 to M.M.M.) and JJCC Castilla-La Mancha and Fondos FEDER (SBPLY/17/180501/000333 to M.L.S.). |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Cellviability
Nanoparticle 02 engineering and technology lcsh:Technology 01 natural sciences Supervivencia celular Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings] lcsh:Chemistry Carbon-based quantum dots Organisms::Eukaryota::Animals [Medical Subject Headings] Semiconductor quantum dots General Materials Science lcsh:QH301-705.5 Instrumentation Fluid Flow and Transfer Processes carbon-based quantum dots semiconductor quantum dots cell viability fibroblasts hepatocytes Chemistry Diseases::Neoplasms::Neoplasms by Site::Digestive System Neoplasms::Liver Neoplasms [Medical Subject Headings] General Engineering 021001 nanoscience & nanotechnology Blood proteins lcsh:QC1-999 Computer Science Applications Chemicals and Drugs::Inorganic Chemicals::Elements::Carbon [Medical Subject Headings] Toxicity Puntos cuánticos 0210 nano-technology Biocompatibility Hepatocitos Context (language use) 010402 general chemistry Technology and Food and Beverages::Technology Industry and Agriculture::Manufactured Materials::Nanostructures::Nanoparticles::Quantum Dots [Medical Subject Headings] Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Survival [Medical Subject Headings] Viability assay Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice [Medical Subject Headings] Diseases::Neoplasms::Neoplasms by Site::Digestive System Neoplasms::Liver Neoplasms::Carcinoma Hepatocellular [Medical Subject Headings] Analytical Diagnostic and Therapeutic Techniques and Equipment::Equipment and Supplies::Electrical Equipment and Supplies::Semiconductors [Medical Subject Headings] lcsh:T Anatomy::Cells::Cells Cultured::Cell Line::3T3 Cells [Medical Subject Headings] Process Chemistry and Technology Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Albumins::Serum Albumin::Serum Albumin Bovine [Medical Subject Headings] Fibroblasts Toxicidad Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Death [Medical Subject Headings] Fibroblastos In vitro 0104 chemical sciences lcsh:Biology (General) lcsh:QD1-999 lcsh:TA1-2040 Hepatocytes Biophysics lcsh:Engineering (General). Civil engineering (General) lcsh:Physics Fetal bovine serum |
| Zdroj: | Applied Sciences; Volume 10; Issue 24; Pages: 8845 Applied Sciences, Vol 10, Iss 8845, p 8845 (2020) |
| ISSN: | 2076-3417 |
| DOI: | 10.3390/app10248845 |
| Popis: | A comparative, fully parallel study of nanoparticles (NPs) toxicity by in vitro cell viability is shown looking for reliable comparability of nanotoxicological results, a well-recognized bottleneck in the context. This procedure is suitable to compare toxicity of similar NPs, as well as the influence on toxicity of the size, surface, and other characteristics. As a case of study, semiconductor (SQDs) and graphitic-carbon quantum dots (CQDs) with identical surface groups and size were evaluated. All experiments were conducted at same conditions, involving two types of cells (mouse fibroblasts (3T3-L1) and carcinoma human hepatocellular cells (HepG2)) and different extracellular components (in the absence or presence of fetal bovine serum (FBS)). Cell viability demonstrated the excellent biocompatibility of CQDs compared to SQDs, which caused higher percentage of cell death at lower concentrations, as predicted but never clearly demonstrated. However, our comparative studies established that the toxicity of SQDs and CQDs are cellular type-dependent, and the absence or presence of serum proteins reduces the minimal concentration necessary of NPs to produce toxicity. |
| Databáze: | OpenAIRE |
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