Sphingomyelin in Human Breast Milk might be Essential for the Hippocampus Maturation
| Autor: | Albi, E., Arcuri, C., Kobayashi, T., Tomishige, N., Dei Cas, M., Paroni, R., Signorelli, P., Cerquiglini, L., Troiani, S., Gizzi, C., Ceccarini, M.R., Mirarchi, A., Cossignani, L., Gil, M.G., Beccari, T., Cataldi, S. |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Cell Nucleus
General Immunology and Microbiology Milk Human human milk Infant General Medicine Hippocampus General Biochemistry Genetics and Molecular Biology sphingomyelin Sphingomyelins neurites cell differentiation Sphingomyelin Phosphodiesterase Milk embryonic hippocampal cells Animals Cattle Female Humans Settore BIO/10 - Biochimica Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica Human |
| Popis: | It has been established that sphingomyelin present human breast milk is useful for the brain maturation and cognitive development. At 10 days of breastfeeding the sphingomyelin content is double that present in cow's milk and its content is independent of the maternal diet. The aim of the study was to analyze the content of sphingomyelin in breast milk at 3 months of breastfeeding and to consider the effect of this molecule on synaptic function and nerve conduction through the probable expansion of myelinated axons.Therefore, to begin to define and assess this, we performed sphingolipidomic analysis in human breast milk. Then, we cultured embryonic hippocampal cells (HN9.10) in the presence of sphingomyelin at a concentration from 0.6% to 31% of human milk, estimated by considering its bioavailability and its passage into the interstitial fluid. To highlight the effect of sphingomyelin in the cells, cell viability and morphology were evaluated. Analyses of neutral sphingomyelinase gene and protein expression was performed. The entry of sphingomyelin into the cell was studied in immunofluorescence; the expression of heavy neurofilament (NF200) was tested with immunocytochemical technique.We demonstrated that sphingomyelin is able to enter cell nucleus and overexpress the sphingomyelin phosphodiesterase 4 (We speculated that the sphingomyelin present in human breast milk is useful in part to regulate nuclear activity and in part to form myelin sheet to facilitate nerve cell maturation. As brain development occurs at 0-3 years, these data open a new avenue of potential intervention to integrate the infant formulas with SM to obtain a product similar to the maternal milk. |
| Databáze: | OpenAIRE |
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