Gene-expression profiling of White spot syndrome virus in vivo
| Autor: | Mariëlle C. W. van Hulten, Hendrik Marks, Adèle van Houwelingen, Oscar Vorst, Just M. Vlak |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Gene Expression Regulation
Viral transcriptional analysis Proteome Transcription Genetic genome sequence White spot syndrome Laboratory of Virology Biology Genome Laboratorium voor Virologie Open Reading Frames Viral Proteins White spot syndrome virus 1 baculovirus dna microarrays Penaeidae Virology Gene expression Animals ORFS Gene Oligonucleotide Array Sequence Analysis Genetics Gene Expression Profiling biology.organism_classification PE&RC proteomic analysis infection proteins Gene expression profiling PRI Bioscience Open reading frame cell-lines identification DNA microarray shrimp |
| Zdroj: | Journal of General Virology 86 (2005) 7 Journal of General Virology, 86(7), 2081-2100 |
| ISSN: | 0022-1317 |
| Popis: | White spot syndrome virus, type species of the genus Whispovirus in the family Nimaviridae, is a large, double-stranded DNA (dsDNA) virus that infects crustaceans. The genome of the completely sequenced isolate WSSV-TH encodes 184 putative open reading frames (ORFs), the functions of which are largely unknown. To study the transcription of these ORFs, a DNA microarray was constructed, containing probes corresponding to nearly all putative WSSV-TH ORFs. Transcripts of 79 % of these ORFs could be detected in the gills of WSSV-infected shrimp (Penaeus monodon). Clustering of the transcription profiles of the individual genes during infection showed two major classes of genes: the first class reached maximal expression at 20 h post-infection (p.i.) (putative early) and the other class at 2 days p.i. (putative late). Nearly all major and minor structural virion-protein genes clustered in the latter group. These data provide evidence that, similar to other large, dsDNA viruses, the WSSV genes at large are expressed in a coordinated and cascaded fashion. Furthermore, the transcriptomes of the WSSV isolates WSSV-TH and TH-96-II, which have differential virulence, were compared at 2 days p.i. The TH-96-II genome encodes 10 ORFs that are not present in WSSV-TH, of which at least seven were expressed in P. monodon as well as in crayfish (Astacus leptodactylus), suggesting a functional but not essential role for these genes during infection. Expression levels of most other ORFs shared by both isolates were similar. Evaluation of transcription profiles by using a genome-wide approach provides a better understanding of WSSV transcription regulation and a new tool to study WSSV gene function. |
| Databáze: | OpenAIRE |
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