DNA double-strand break repair functions defend against parvovirus infection
| Autor: | Solon L. Rhode, M H Schneiderman, T J Tauer, Jamboor K. Vishwanatha |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
DNA Repair
DNA damage DNA repair Cell Survival viruses Immunology CHO Cells Biology Virus Replication Microbiology Genome Parvovirus chemistry.chemical_compound Virology Cricetinae Animals Cell Nucleus Chinese hamster ovary cell virus diseases Dose-Response Relationship Radiation biology.organism_classification Molecular biology Double Strand Break Repair chemistry Viral replication Insect Science Receptors Virus DNA Research Article |
| Zdroj: | Journal of virology. 70(9) |
| ISSN: | 0022-538X |
| Popis: | The majority of human cancers appear to arise from mutations caused by DNA damage (5). One significant source of mutations is error-prone repair of DNA double-strand breaks (DSB). DSB can result from normal oxidative metabolism, replication errors, and exposure to X-rays. Cells eliminate most of this damage through DNA repair pathways by homologousrecombinationornonhomologousend-joiningpathways. The latter are more heavily utilized in mammalian cells and more error prone. These repair pathways are essential for maintenance of the overall integrity of the DNA genome. Mammalian cells that are defective in repairing DSB are significantly more sensitive to X-rays than are their normal counterparts. A number of cell lines with defective DNA DSB repair and V(D)J recombination have been established from Chinese hamster ovary (CHO) cells (23). V(D)J recombination is necessary for normal B- and T-cell immunity, and these specialized forms of DNA rearrangements interface with the system for generic DNA break repair. Defects in this recombination pathway can result in severe combined immune deficiency and sensitivity to ionizing radiation (17). Because parvoviruses have a linear DNA genome (3), we investigated what role DNA repair functions may play in parvovirus infection. We hypothesized that the linear viral genomes may be substrates for the DNA repair machinery. For |
| Databáze: | OpenAIRE |
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