Eucalyptol alleviates inflammation and pain responses in a mouse model of gout arthritis

Autor:	Ping Wang, Xiaojie Li, Yuanyuan Li, Boyu Liu, Boyi Liu, Chuan Wang, Chengyu Yin, Xiaoli Zheng, Yan Tai
Rok vydání:	2020
Předmět:	0301 basic medicine Gout medicine.medical_treatment TRPV1 Pain Inflammation Pharmacology medicine.disease_cause Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo medicine Animals Humans Eucalyptol business.industry Macrophages Inflammasome Research Papers Uric Acid 030104 developmental biology medicine.anatomical_structure Cytokine chemistry Quality of Life medicine.symptom Ankle business 030217 neurology & neurosurgery Oxidative stress medicine.drug
Zdroj:	Br J Pharmacol
ISSN:	1476-5381 0007-1188
Popis:	Background and purpose Gout arthritis, which is provoked by monosodium urate (MSU) crystal accumulation in the joint and periarticular tissues, induces severe pain and affects quality of life of the patients. Eucalyptol (1,8-cineol), the principal component in the essential oils of eucalyptus leaves, is known to possess anti-inflammatory and analgesic properties. We aimed to examine the therapeutic effects of eucalyptol on gout arthritis and related mechanisms. Experimental approach A mouse model of gout arthritis was established via MSU injection into the ankle joint. Ankle oedema, mechanical allodynia, neutrophil infiltration, oxidative stress, NLRP3 inflammasome, and TRPV1 expression were examined. Key results Eucalyptol attenuated MSU-induced mechanical allodynia and ankle oedema in dose-dependently, with effectiveness similar to indomethacin. Eucalyptol reduced inflammatory cell infiltrations in ankle tissues. Eucalyptol inhibited NLRP3 inflammasome activation and pro-inflammatory cytokine production induced by MSU in ankle tissues in vivo. Eucalyptol reduced oxidative stress induced by MSU in RAW264.7 cells in vitro as well as in ankle tissues in vivo, indicated by an increase in activities of antioxidant enzymes and reduction of ROS. Eucalyptol attenuated MSU-induced up-regulation of TRPV1 expression in ankle tissues and dorsal root ganglion neurons innervating the ankle. The in vivo effects of eucalyptol on ankle oedema, mechanical allodynia, NLRP3 inflammasome, IL-1β, and TRPV1 expression were mimicked by treating MSU-injected mice with antioxidants. Conclusion and implications Eucalyptol alleviates MSU-induced pain and inflammation via mechanisms possibly involving anti-oxidative effect. Eucalyptol and other antioxidants may represent promising therapeutic options for gout arthritis.
Databáze:	OpenAIRE
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