Diagnostic DNA-Flow- vs. -Image-Cytometry in Effusion Cytology
| Autor: | Thomas Heiden, Motherby H, Mary Kube, Alfred Böcking, Natalia Pomjanski, Bernhard Tribukait, Alexandra Boros |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Mesothelioma
Pathology medicine.medical_specialty lcsh:RC254-282 Epithelium Flow cytometry Serous Membrane cytopathology Cytology Carcinoma Biomarkers Tumor Medicine Ascitic Fluid Humans lcsh:QH573-671 Image Cytometry medicine.diagnostic_test business.industry lcsh:Cytology Serous membrane Reproducibility of Results DNA medicine.disease Aneuploidy Flow Cytometry lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Pleural Effusion Malignant Serous fluid medicine.anatomical_structure Editorial Effusion Data Interpretation Statistical serous effusion DNA‐image‐cytometry Other business Cytometry Algorithms DNA‐flow‐cytometry |
| Zdroj: | Analytical Cellular Pathology : the Journal of the European Society for Analytical Cellular Pathology Analytical Cellular Pathology, Vol 24, Iss 1, Pp 5-15 (2002) |
| ISSN: | 1878-3651 0921-8912 |
| Popis: | Aims: To determine the sensitivity and specificity of flow‐ and image‐cytometry for the detection of DNA‐aneuploidy as a marker for malignant cells in effusions.Methods: 200 effusions (80 tumor cell‐positive, 74 negative and 46 cytologically equivocal) were stained with DAPI‐SR for DNA‐flow‐ and with Feulgen‐Pararosaniline for ‐image‐cytometry. They were measured using a PAS‐flow‐cytometer and an AutoCyte‐QUIC‐DNA‐workstation according to the ESACP consensus reports for DNA‐flow‐ and ‐image‐cytometry, respectively [7,23,29,49].Results: Sensitivity of DNA‐aneuploidy for the identification of malignant cells was 32.1% for DNA‐flow‐ and 75.0% for ‐image‐cytometry, specificity of ‐euploidy in benign cells was 100.0% for both methods. Positive predictive value of DNA‐aneuploidy for the identification of malignant cells was 100.0% for both techniques, negative predictive value of DNA‐euploidy was 48.6% for DNA‐flow‐ and 72.0% for ‐image‐cytometry.Conclusions: Searching for DNA‐aneuploidy as a diagnostic marker for neoplastic cells in serous effusions image‐cytometry revealed superior sensitivity as compared with monoparametric flow cytometry. |
| Databáze: | OpenAIRE |
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