Bladder cancer detection by urinary extracellular vesicle mRNA analysis
| Autor: | Hiroshi Tanaka, Taku Murakami, Takahiro Osawa, Hiroshi Harada, Tomoshige Akino, Toshimori Seki, Nobuyuki Fukuzawa, Hidetaka Suzuki, Takahiro Tsuji, Cindy Yamamoto |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty mRNA Urinary system Urology urologic and male genital diseases Exosome 03 medical and health sciences 0302 clinical medicine Ureter exosome Medicine Bladder cancer business.industry Cancer Extracellular vesicle medicine.disease 030104 developmental biology medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis bladder cancer biomarker Biomarker (medicine) extracellular vesicles business Renal pelvis Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| DOI: | 10.18632/oncotarget.25998 |
| Popis: | // Taku Murakami 1 , Cindy M. Yamamoto 1 , Tomoshige Akino 2 , Hiroshi Tanaka 2 , Nobuyuki Fukuzawa 2 , Hidetaka Suzuki 2 , Takahiro Osawa 3 , Takahiro Tsuji 2 , Toshimori Seki 2 and Hiroshi Harada 2 1 Hitachi Chemical Co. America, Ltd., Irvine, CA, USA 2 Sapporo City General Hospital, Sapporo, Japan 3 Graduate School of Medicine, Hokkaido University, Sapporo, Japan Correspondence to: Taku Murakami, email: tmurakami@hitachi-chemical.com Keywords: bladder cancer; biomarker; extracellular vesicles; exosome; mRNA Received: May 03, 2018 Accepted: August 04, 2018 Published: August 28, 2018 ABSTRACT Objective: Urinary extracellular vesicles (EV) could be promising biomarkers for urological diseases. In this retrospective feasibility study, we conducted biomarker screening for early stage bladder cancer using EV mRNA analysis. Methods: Biomarker candidates were identified through RNA-seq analysis of urinary EV from patients with non-muscle invasive bladder cancer (N=3), advanced urothelial cancer (N=3), no residual tumor after TURBT (N=2), and healthy and disease controls (N=4). Diagnostic performance was evaluated by RT-qPCR in a larger patient group including bladder cancer (N=173), renal pelvis and ureter cancer (N=33), no residual tumor and non-cancer disease control (N=36). Results: Urinary EV SLC2A1 , GPRC5A and KRT17 were overexpressed in pT1 and higher stage bladder cancer by 20.6-fold, 18.2-fold and 29.5-fold, respectively. These genes allowed detection of non-muscle invasive bladder cancer (AUC: 0.56 to 0.64 for pTa, 0.62 to 0.80 for pTis, and 0.82 to 0.86 for pT1) as well as pT2 and higher muscle invasive bladder cancer (AUC: 0.72 to 0.90). Subgroup analysis indicated that these markers could be useful for the detection of cytology-negative/-suspicious and recurrent bladder cancers. Conclusion: Three urinary EV mRNA were discovered to be elevated in bladder cancer. Urinary EV mRNA are promising biomarkers of urothelial cancer and worth further investigation. |
| Databáze: | OpenAIRE |
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