Co-ordinated expression of innate immune molecules during mouse neurulation

Autor: Angela Jeanes, Susanna Mantovani, Trent M. Woodruff, Kathryn Markham, Liam G. Coulthard
Rok vydání: 2015
Předmět:
Complement Pathway
Alternative
Receptor for Advanced Glycation End Products
Immunology
Complement receptor
Biology
RAGE (receptor)
Mice
03 medical and health sciences
Classical complement pathway
0302 clinical medicine
Animals
Protein Isoforms
Complement Pathway
Classical
Neurulation
Molecular Biology
In Situ Hybridization
030304 developmental biology
0303 health sciences
TNF Receptor-Associated Factor 4
Innate immune system
Toll-Like Receptors
Innate lymphoid cell
Pattern recognition receptor
CCL18
Gene Expression Regulation
Developmental
Complement Pathway
Mannose-Binding Lectin
Complement System Proteins
Embryo
Mammalian
MAP Kinase Kinase Kinases
Immunity
Innate
Cell biology
Mice
Inbred C57BL
Myeloid Differentiation Factor 88
Complement membrane attack complex
030217 neurology & neurosurgery
Signal Transduction
Zdroj: Molecular Immunology. 68:253-260
ISSN: 0161-5890
Popis: The innate immune system is the first line of defence against pathogens and infection. Recently, it has become apparent that many innate immune factors have roles outside of immunity and there is growing evidence that these factors play important functional roles during the development of a range of model organisms. Several studies have documented developmental expression of individual factors of the toll-like receptor and complement systems, and we recently demonstrated a key role for complement C5a receptor (C5aR1) signalling in neural tube closure in mice. Despite these emerging studies, a comprehensive expression analysis of these molecules in embryonic development is lacking. In the current study, we therefore, examined the expression of key innate immune factors in the early development period of neurulation (7.5-10.5dpc) in mice. We found that complement factor genes were differentially expressed during this period of murine development. Interestingly, the expression patterns we identified preclude activation of the classical and alternative pathways and formation of the membrane attack complex. Additionally, several other classes of innate immune molecules were expressed during the period of neurulation, including toll-like receptors (TLR-2, -3, -4 and -9), receptor for advanced glycation end-products (RAGE), and their signalling adapters (TRAF-4, TRAF-6, TAK-1 and MyD88). Taken together, this study highlights a number of innate immune factors as potential novel players in early embryonic development.
Databáze: OpenAIRE
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