Long intergenic non-coding RNA APOC1P1-3 inhibits apoptosis by decreasing α-tubulin acetylation in breast cancer
| Autor: | Yu Jg, Zou Q, Daibing Zhou, Jimeng Yang, Jiyan Wang, Li Li, Li-Hua Lv, Ren Kh, Liao Xh, Yinpeng Jin, Pinghong Zhou, Xinhua Liu, Lu Q, Yu J |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Cancer Research Immunology Breast Neoplasms Biology 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Breast cancer Tubulin Cell Line Tumor medicine Humans RNA Small Interfering Promoter Regions Genetic Cell Proliferation Neoplasm Staging Regulation of gene expression Caspase 3 Gene Expression Profiling RNA Promoter Acetylation Cell Biology DNA Methylation Middle Aged medicine.disease Non-coding RNA Microarray Analysis Molecular biology Gene Expression Regulation Neoplastic 030104 developmental biology Tumor progression 030220 oncology & carcinogenesis DNA methylation Cancer research Original Article Female RNA Long Noncoding Signal Transduction |
| Zdroj: | Cell Death & Disease |
| ISSN: | 2041-4889 |
| Popis: | Increasing evidence indicates that long non-coding RNAs (lncRNAs) act as important regulatory factors in tumor progression. However, their roles in breast cancer remain largely unknown. In present studies, we identified aberrantly expressed long intergenic non-coding RNA APOC1P1-3 (lincRNA-APOC1P1-3) in breast cancer by microarray, verified it by quantitative real-time PCR, and assessed methylation status in the promoter region by pyrosequencing. We also investigated the biological functions with plasmid transfection and siRNA silencing experiments, and further explored their mechanisms by RNA pull-down and RNA immunoprecipitation to identify binding proteins. We found that 224 lncRNAs were upregulated in breast cancer, whereas 324 were downregulated. The lincRNA-APOC1P1-3 was overexpressed in breast cancer, which was related to tumor size and hypomethylation in its promoter region. We also found that APOC1P1-3 could directly bind to tubulin to decrease α-tubulin acetylation, to inactivate caspase-3, and to inhibit apoptosis. This study demonstrates that overexpression of APOC1P1-3 can inhibit breast cancer apoptosis. |
| Databáze: | OpenAIRE |
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