Production and partial characterization of dehairing protease from Bacillus cereus MCM B-326
Autor: | Pradnya P. Kanekar, Vasudeo Zambare, S.S. Sarnaik, Prashant K. Dhakephalkar, S. S. Nilegaonkar |
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Rok vydání: | 2007 |
Předmět: |
Proteases
Environmental Engineering Starch medicine.medical_treatment Soybean meal Bacillus cereus Bioengineering Substrate Specificity chemistry.chemical_compound medicine Ammonium Waste Management and Disposal chemistry.chemical_classification Chromatography Protease biology Renewable Energy Sustainability and the Environment Temperature General Medicine Hydrogen-Ion Concentration biology.organism_classification Bacillales Enzyme chemistry Biochemistry Electrophoresis Polyacrylamide Gel Peptide Hydrolases |
Zdroj: | Bioresource Technology. 98:1238-1245 |
ISSN: | 0960-8524 |
DOI: | 10.1016/j.biortech.2006.05.003 |
Popis: | Bacillus cereus MCM B-326, isolated from buffalo hide, produced an extracellular protease. Maximum protease production occurred (126.87+/-1.32 U ml(-1)) in starch soybean meal medium of pH 9.0, at 30 degrees C, under shake culture condition, with 2.8 x 10(8) cells ml(-1) as initial inoculum density, at 36 h. Ammonium sulphate precipitate of the enzyme was stable over a temperature range of 25-65 degrees C and pH 6-12, with maximum activity at 55 degrees C and pH 9.0. The enzyme required Ca(2+) ions for its production but not for activity and/or stability. The partially purified enzyme exhibited multiple proteases of molecular weight 45 kDa and 36 kDa. The enzyme could be effectively used to remove hair from buffalo hide indicating its potential in leather processing industry. |
Databáze: | OpenAIRE |
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