Decreased spinal cord content of calcitonin gene-related peptide in the spontaneously hypertensive rat
| Autor: | Jay Carson, Karin N. Westlund, Donald J. DiPette, O. Bryan Holland |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
medicine.medical_specialty Calcitonin Gene-Related Peptide Central nervous system chemistry.chemical_element Substance P Blood Pressure Calcitonin gene-related peptide Calcium Rats Inbred WKY chemistry.chemical_compound Spontaneously hypertensive rat Internal medicine Rats Inbred SHR medicine Animals Homeostasis Calcium metabolism Neurons business.industry General Neuroscience Body Weight Spinal cord Immunohistochemistry Rats Calcium Dietary Endocrinology medicine.anatomical_structure nervous system chemistry Spinal Cord Calcitonin Hypertension business |
| Zdroj: | Neuroscience letters. 131(2) |
| ISSN: | 0304-3940 |
| Popis: | Calcitonin gene-related peptide (CGRP), produced by alternative processing of the primary transcript of the calcitonin gene, is a potent vasodilator. We have shown that dietary calcium deficiency accompanied by decreased serum ionized calcium significantly decreases the neuronal content of CGRP in laminae I and II of the dorsal horn of the spinal cord in the growing rat. The spontaneously hypertensive rat (SHR) is characterized by decreased serum ionized calcium levels and is thought to most closely resemble human essential hypertension. To determine if the neuronal content of CGRP is decreased in the SHR compared to the Wistar-Kyoto (WKY) parent strain, CGRP was localized immunocytochemically in the dorsal horn of the spinal cord. The density of immunocytochemical staining was quantitated by computer-assisted image processing of laminae I and II of the upper thoracic spinal cord of 12-14 week old male SHR (n = 4) and WKY (n = 4) normotensive, control rats. The SHR had significantly decreased neuronal CGRP content compared to the WKY rats (107 +/- 5 vs 121 +/- 6 arbitrary units, P less than 0.01). In contrast, the neuronal density of substance P (SP), which frequently co-exists with CGRP in this neuronal population, was not different between the two groups (SHR, 91 +/- 6 (n = 4) vs WKY, 88 +/- 3 arbitrary units (n = 4)). |
| Databáze: | OpenAIRE |
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