Detection and Estimation of alpha-Amyrin, beta-Sitosterol, Lupeol, and n-Triacontane in Two Medicinal Plants by High Performance Thin Layer Chromatography
| Autor: | Vidya Dighe, Saikat S. Mallick |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
beta-Sitosterol
Chromatography Pluchea lanceolata Article Subject Silica gel Mechanical Engineering Energy Engineering and Power Technology Management Science and Operations Research lcsh:Chemistry chemistry.chemical_compound lcsh:QD1-999 chemistry Alpha-amyrin High performance thin layer chromatography Derivatization Medicinal plants Lupeol |
| Zdroj: | Advances in Chemistry, Vol 2014 (2014) |
| ISSN: | 2356-6612 |
| DOI: | 10.1155/2014/143948 |
| Popis: | A normal phase high performance thin layer chromatography (HPTLC) method has been developed and validated for simultaneous estimation of four components, namely, alpha-amyrin, beta-sitosterol, lupeol, and n-triacontane from two medicinally important plants, Leptadenia reticulata Wight & Arn. and Pluchea lanceolata (DC.) CB. Clarke. In Ayurveda, both plants have been reported to possess immunomodulatory activity. Chromatographic separation of the four components from the methanolic extracts of whole plant powders of Leptadenia reticulata Wight & Arn. and Pluchea lanceolata (DC.) CB. Clarke. was performed on TLC aluminium plates precoated with silica gel 60F254 using a suitable mobile phase. The densitometric scanning was done after derivatization at λ = 580 nm for α-amyrin, β-sitosterol, and lupeol, and at 366 nm for n-triacontane. The developed HPTLC method has been validated and used for simultaneous quantitation of the four components from the methanolic extracts of whole plant powders of Leptadenia reticulata Wight & Arn. and Pluchea lanceolata (DC.) CB. Clarke. The developed HPTLC method is simple, rapid, and precise and can be used for routine quality control. |
| Databáze: | OpenAIRE |
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