Supplementary video 1 from Tumor-Produced Interleukin-8 Attracts Human Myeloid-Derived Suppressor Cells and Elicits Extrusion of Neutrophil Extracellular Traps (NETs)

Autor: Ignacio Melero, Jose L. Perez-Gracia, Pedro Berraondo, Jose Medina-Echeverz, Marcello Allegretti, Maria De Pizzol, Susana Inogés, Angela Aznar, Juan P. Fusco, Saray Garasa, Alfonso Rodriguez-Paulete, Arantza Azpilikueta, Sara Labiano, Diego Alignani, Maria Pilar Andueza, Miguel F. Sanmamed, Guiomar Pérez, Carmen Oñate, Alvaro Teijeira, Carlos Alfaro
Rok vydání: 2023
DOI: 10.1158/1078-0432.22457468
Popis: Supplementary video 1. IL-8 induces NET extrusion of GrMDSCs using time-lapse in vivo confocal fluorescence microsocopy. GrMDSCs nuclei were pre-stained with DRAQ5 (Red) and treated with IL-8. The non-cell permeant green DNA probe (SYTOXgreen) is present in the culture media and only stains DNA when is extruded extracelullarly. Video represent over five hours a maximum intensity projection of a Zstack. Video is 600x accelerated
Databáze: OpenAIRE