EXPRESS: Optimization of Sample Preparation Using Glass Slides for Spectral Pathology
| Autor: | Ibraheem Yousef, Deborah Latham, Srinivas Pillai, Lewis M. Dowling, Paul D. Roach, Abigail V. Rutter, Josep Sulé-Suso, Daniel Gey van Pittius |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty Materials science Lung Neoplasms Infrared Translation (geometry) Q1 01 natural sciences Range finding Specimen Handling 03 medical and health sciences symbols.namesake Fourier transform infrared Notes Cell Line Tumor Spectroscopy Fourier Transform Infrared medicine Humans Sample preparation Fourier transform infrared spectroscopy Spectroscopy Instrumentation 030304 developmental biology 0303 health sciences microspectroscopy Microscopy 010401 analytical chemistry smear Histological Techniques cytospin R735 glass coverslips cell fixation R1 0104 chemical sciences FT-IR lung cancer Fourier transform Applied spectroscopy symbols RB RC |
| Zdroj: | Applied Spectroscopy |
| ISSN: | 1943-3530 0003-7028 |
| Popis: | The clinical translation of Fourier transform infrared (FT-IR) microspectroscopy in pathology will require bringing this technique as close as possible to standard practice in pathology departments. An important step is sample preparation for both FT-IR microspectroscopy and pathology. This should entail minimal disruption of standard clinical practice while achieving good quality FT-IR spectral data. In fact, the recently described possibility of obtaining FT-IR spectra of cells placed on glass substrates brings FT-IR microspectroscopy closer to a clinical application. We have now furthered this work in order to identify two different types of lung cancer cells placed on glass coverslips. Two types of sample preparation which are widely used in pathology, cytospin and smear, have been used. Samples were fixed with either methanol, used in pathology, or formalin (4% paraformaldehyde) used widely in spectroscopy. Fixation with methanol (alcohol-based fixative) removed lipids from cells causing a decrease in intensity of the peaks at 2850 cm−1 and 2920 cm−1. Nevertheless, we show for the first time that using either type of sample preparation and fixation on thin glass coverslips allowed to differentiate between two different types of lung cancer cells using either the lipid region or the fingerprint region ranging from 1800 cm−1 to 1350 cm−1. We believe that formalin-fixed cytospin samples would be preferred to study cells on thin coverslips using FT-IR microspectroscopy. This work presents a clear indication for future advances in clinical assessment of samples within pathology units to gain a deeper understanding of cells/tissues under investigation. |
| Databáze: | OpenAIRE |
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