Reduced expressions of calmodulin genes and protein and reduced ability of calmodulin to activate plasma membrane Ca(2+)-ATPase in the brain of protein undernourished rats: modulatory roles of selenium and zinc supplementation
| Autor: | Alka Khera, Gbenga A. Adenuga, Olusegun L. Adebayo, Rajat Sandhir |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male Calmodulin ATPase Clinical Biochemistry chemistry.chemical_element Calcium Biochemistry Sarcoplasmic Reticulum Calcium-Transporting ATPases 03 medical and health sciences Selenium 0302 clinical medicine Casein Gene expression Animals Rats Wistar Synaptosome biology Gene Expression Profiling Cell Membrane Malnutrition Brain Cell Biology General Medicine Cortex (botany) Rats Zinc 030104 developmental biology chemistry Dietary Supplements biology.protein 030217 neurology & neurosurgery |
| Zdroj: | Cell biochemistry and function. 34(2) |
| ISSN: | 1099-0844 |
| Popis: | The roles of protein undernutrition as well as selenium (Se) and zinc (Zn) supplementation on the ability of calmodulin (CaM) to activate erythrocyte ghost membrane (EGM) Ca(2+)-ATPase and the calmodulin genes and protein expressions in rat's cortex and cerebellum were investigated. Rats on adequate protein diet and protein-undernourished (PU) rats were fed with diet containing 16% and 5% casein, respectively, for a period of 10 weeks. The rats were then supplemented with Se and Zn at a concentration of 0.15 and 227 mg l(-1), respectively, in drinking water for 3 weeks. The results obtained from the study showed significant reductions in synaptosomal plasma membrane Ca(2+)-ATPase (PMCA) activity, Ca(2+)/CaM activated EGM Ca(2+) ATPase activity and calmodulin genes and protein expressions in PU rats. Se or Zn supplementation improved the ability of Ca(2+)/CaM to activate EGM Ca(2+)-ATPase and protein expressions. Se or Zn supplementation improved gene expression in the cerebellum but not in the cortex. Also, the activity of PMCA was significantly improved by Zn. In conclusion, it is postulated that Se and Zn might be beneficial antioxidants in protecting against neuronal dysfunction resulting from reduced level of calmodulin such as present in protein undernutrition. |
| Databáze: | OpenAIRE |
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