Fork head and Sage maintain a uniform and patent salivary gland lumen through regulation of two downstream target genes,PH4αSG1andPH4αSG2
| Autor: | Whitney K. Mihoulides, Deborah J. Andrew, Elliott W. Abrams |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
medicine.medical_specialty
Procollagen-Proline Dioxygenase Morphogenesis Electrophoretic Mobility Shift Assay Biology Cyclic AMP Response Element-Binding Protein A Salivary Glands Microscopy Electron Transmission Internal medicine Basic Helix-Loop-Helix Transcription Factors medicine Animals Drosophila Proteins Salivary Proteins and Peptides Molecular Biology Transcription factor In Situ Hybridization Tube formation Microscopy Confocal Salivary gland Gene Expression Regulation Developmental Nuclear Proteins Forkhead Transcription Factors Immunohistochemistry Tube closure Phenotype Cell biology Drosophila melanogaster Endocrinology Secretory protein medicine.anatomical_structure Tube morphogenesis Transcription Factors Developmental Biology |
| Zdroj: | Development. 133:3517-3527 |
| ISSN: | 1477-9129 0950-1991 |
| Popis: | (Fkh) is required to block salivary gland apoptosis, internalize salivary gland precursors, prevent expression of duct genes in secretory cells and maintain expression of CrebA, which is required for elevated secretory function. Here, we characterize two new Fkh-dependent genes: PH4alphaSG1 and PH4alphaSG2. We show through in vitro DNA-binding studies and in vivo expression assays that Fkh cooperates with the salivary gland-specific bHLH protein Sage to directly regulate expression of PH4alphaSG2, as well as sage itself, and to indirectly regulate expression of PH4alphaSG1. PH4alphaSG1 and PH4alphaSG2 encode alpha-subunits of resident ER enzymes that hydroxylate prolines in collagen and other secreted proteins. We demonstrate that salivary gland secretions are altered in embryos missing function of PH4alphaSG1 and PH4alphaSG2; secretory content is reduced and shows increased electron density by TEM. Interestingly, the altered secretory content results in regions of tube dilation and constriction, with intermittent tube closure. The regulation studies and phenotypic characterization of PH4alphaSG1 and PH4alphaSG2 link Fkh, which initiates tube formation, to the maintenance of an open and uniformly sized secretory tube. |
| Databáze: | OpenAIRE |
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