Comparison of Dantrolene Sodium with Erythromycin Estolate Using Primary Cultures of Rat Hepatocytes
| Autor: | Daniel Acosta, Elsie M. B. Sorensen |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Erythromycin Estolate
medicine.medical_specialty Cell Survival Health Toxicology and Mutagenesis Biology Toxicology Dantrolene Dantrolene Sodium chemistry.chemical_compound Lactate dehydrogenase Internal medicine Parenchyma medicine Animals Cytotoxic T cell Elevated ldh Cells Cultured Pharmacology Chemical Health and Safety L-Lactate Dehydrogenase Public Health Environmental and Occupational Health Hepatotoxin Rats Inbred Strains General Medicine Erythromycin Rats medicine.anatomical_structure Endocrinology Liver chemistry Biochemistry Hepatocyte Chemical and Drug Induced Liver Injury |
| Zdroj: | Drug and Chemical Toxicology. 8:219-237 |
| ISSN: | 1525-6014 0148-0545 |
| DOI: | 10.3109/01480548509038647 |
| Popis: | Using primary cultures of parenchymal hepatocytes as a model system, the cytotoxic potential of dantrolene sodium (DS) was compared with that of erythromycin estolate (EE)--a known hepatotoxin. Parallel morphological and functional comparisons were made, following 4-, 8-, or 24-h exposures of hepatocyte cultures, using phase contrast microscopy and lactate dehydrogenase (LDH) leakage, respectively. Four-hour exposures of cultures to rather low concentrations of EE (i.e. 50 microM) resulted in cellular necrosis and significantly elevated LDH release. As the concentration of this hepatotoxin was increased, the changes were more pronounced. However, even 4- or 8-h exposures of cultures to a maximum of 100 microM DS did not affect LDH leakage or morphological integrity, although marginally detectable morphological changes did not occur at the highest concentration after 24-h. The value of using primary parenchymal hepatocyte cultures as a model system for the assessment of xenobiotic-induced hepatotoxicity was confirmed. |
| Databáze: | OpenAIRE |
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