Effects of Supernatants from Escherichia coli Nissle 1917 and Faecalibacterium prausnitzii on Intestinal Epithelial Cells and a Rat Model of 5-Fluorouracil-Induced Mucositis

Autor:	Hanru Wang, Susan E.P. Bastian, Yoga D. Jatmiko, Suzanne Mashtoub, Gordon S. Howarth
Rok vydání:	2017
Předmět:	Mucositis 0301 basic medicine Cancer Research Cell Survival Medicine (miscellaneous) Faecalibacterium prausnitzii Microbiology 03 medical and health sciences In vivo Escherichia coli Animals Humans Immunologic Factors Medicine Viability assay Nutrition and Dietetics biology Tight junction business.industry Probiotics Anti-Inflammatory Agents Non-Steroidal Epithelial Cells medicine.disease biology.organism_classification Molecular biology In vitro Rats Intestines 030104 developmental biology Oncology Caco-2 Culture Media Conditioned Myeloperoxidase biology.protein Female Fluorouracil Caco-2 Cells business
Zdroj:	Nutrition and Cancer. 69:307-318
ISSN:	1532-7914 0163-5581
DOI:	10.1080/01635581.2017.1263747
Popis:	Faecalibacterium prausnitzii (Fp) and Escherichia coli Nissle 1917 (EcN) are probiotics, which have been reported to ameliorate certain gastrointestinal disorders. We evaluated the effects of supernatants (SN) derived from Fp and EcN on 5-fluorouracil (5-FU)-treated intestinal cells and in a rat model of mucositis. In vitro: IEC-6, Caco-2, and T-84 cells were analyzed for viability and monolayer permeability. In vivo: Female dark agouti rats were gavaged with Fp or EcN SN and injected intraperitoneally with saline (control) or 5-FU to induce mucositis. Rats were euthanized and intestinal tissues collected for myeloperoxidase assay and histological analyses. In vitro: Caco-2 cell viability was further reduced when treated with Fp SN + 5-FU compared to 5-FU controls. In both Caco-2 and T-84 cells, Fp SN partially prevented the decrease in transepithelial electrical resistance (TER) caused by 5-FU administration. In vivo: 5-FU-injected rats administered Fp SN or EcN SN partly prevented body weight loss and normalized water intake compared to 5-FU controls. These results suggest a growth inhibitory mechanism of Fp SN action on transformed epithelial cells that could be mediated by effects on tight junctions. Factors derived from Fp SN and EcN SN could have a role in reducing the severity of intestinal mucositis.
Databáze:	OpenAIRE
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