Expression of bacteriocin LsbB is dependent on a transcription terminator
| Autor: | Milan Kojic, Natasa Tosic, Gordana Uzelac, Marija Miljkovic, Jelena Lozo, Zorica Radulovic |
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| Rok vydání: | 2015 |
| Předmět: |
DNA
Bacterial Transcription Genetic Microbiology Gene Knockout Techniques 03 medical and health sciences Plasmid Bacteriocins Bacteriocin LsbB baceriocin Promoter Regions Genetic Gene 030304 developmental biology Terminator Regions Genetic Genetics 0303 health sciences biology 030306 microbiology Lactococcus lactis Gene Expression Regulation Bacterial biology.organism_classification Molecular biology Terminator (genetics) Subcloning Genes Bacterial Transcription terminator regulation Class II bacteriocin Heterologous expression Bacteriocin expression Plasmids |
| Zdroj: | Microbiological Research |
| Popis: | The production of LsbB, leaderless class II bacteriocin, is encoded by genes (lsbB and lmrB) located on plasmid pMN5 in Lactococcus lactis BGMN1-5. Heterologous expression of the lsbB gene using the pAZIL vector (pAZIL-lsbB) in L. lactis subsp. cremoris MG7284 resulted in a significant reduction (more than 30 times) of bacteriocin LsbB expression. Subcloning and deletion experiments with plasmid pMN5 revealed that full expression of LsbB requires the presence of a complete transcription terminator located downstream of the lsbB gene. RNA stability analysis revealed that the presence of a transcription terminator increased the RNA stability by three times and the expression of LsbB by 30 times. The study of the influence of transcription terminator on the expression of other bacteriocin genes (lcnB, for lactococcin B production) indicated that this translational terminator likely functions in a lsbB-specific manner rather than in a general manner. |
| Databáze: | OpenAIRE |
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