Transcriptomics of Haemophilus (Glässerella) parasuis serovar 5 subjected to culture conditions partially mimetic to natural infection for the search of new vaccine antigens
| Autor: | Sonia Martínez-Martínez, César B. Gutiérrez-Martín, Álvaro Álvarez-Estrada, Elías F. Rodríguez-Ferri |
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| Přispěvatelé: | Sanidad Animal, Facultad de Veterinaria |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Serotype Haemophilus (Glässerella) parasuis Vacunas Haemophilus Infections Swine Vaccine antigens Sanidad animal 030106 microbiology RNA-sequencing Glässer’s disease Ganado porcino Microbiology Transcriptome 03 medical and health sciences Haemophilus parasuis Antigen Haemophilus Animals Gene Haemophilus Vaccines Swine Diseases Antigens Bacterial lcsh:Veterinary medicine General Veterinary biology Sequence Analysis RNA Gene Expression Profiling General Medicine biology.organism_classification Iron uptake Tetratricopeptide 030104 developmental biology biology.protein lcsh:SF600-1100 Antibody Veterinaria Bacterial outer membrane Research Article |
| Zdroj: | BMC Veterinary Research, Vol 14, Iss 1, Pp 1-11 (2018) BMC Veterinary Research |
| Popis: | Background Haemophilus (Glässerella) parasuis is the etiological agent of Glässer’s disease in pigs. Control of this disorder has been traditionally based on bacterins. The search for alternative vaccines has focused mainly on the study of outer membrane proteins. This study investigates the transcriptome of H. (G.) parasuis serovar 5 subjected to in vitro conditions mimicking to those existing during an infection (high temperature and iron-restriction), with the aim of detecting the overexpression of genes coding proteins exposed on bacterial surface, which could represent good targets as vaccine candidates. Results The transcriptomic approach identified 13 upregulated genes coding surface proteins: TbpA, TbpB, HxuA, HxuB, HxuC, FhuA, FimD, TolC, an autotransporter, a protein with immunoglobulin folding domains, another large protein with a tetratricopeptide repeat and two small proteins that did not contain any known domains. Of these, the first six genes coded proteins being related to iron extraction. Conclusion Six of the proteins have already been tested as vaccine antigens in murine and/or porcine infection models and showed protection against H. (G.) parasuis. However, the remaining seven have not yet been tested and, consequently, they could become useful as putative antigens in the prevention of Glässer’s disease. Anyway, the expression of this seven novel vaccine candidates should be shown in other serovars different from serovar 5. Electronic supplementary material The online version of this article (10.1186/s12917-018-1647-1) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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