Mus cervicolor Murine Leukemia Virus Isolate M813 Belongs to a Unique Receptor Interference Group
| Autor: | Carol Stocking, Marion Ziegler, Carsten Münk, Vladimir S. Prassolov, Sibyll Hein, Dmitry Ivanov, Jürgen Löhler |
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| Rok vydání: | 2001 |
| Předmět: |
Genes
Viral Sequence analysis viruses Molecular Sequence Data Immunology Clone (cell biology) Biology Virus Replication Southeast asian Microbiology Virus Mice hemic and lymphatic diseases Virology Viral Interference Murine leukemia virus Animals Humans Coding region Amino Acid Sequence Cloning Molecular Cells Cultured Mus cervicolor Genetics Base Sequence Sequence Homology Amino Acid Gene Products env Membrane Proteins Chromosome 3T3 Cells Sequence Analysis DNA biochemical phenomena metabolism and nutrition biology.organism_classification Rats Virus-Cell Interactions Leukemia Virus Murine Muridae Tumor Virus Infections Mutagenesis Insect Science DNA Viral Amino Acid Transport Systems Basic Receptors Virus Moloney murine leukemia virus Carrier Proteins HeLa Cells Retroviridae Infections |
| Zdroj: | Journal of Virology. 75:4490-4498 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.75.10.4490-4498.2001 |
| Popis: | Murine leukemia virus (MuLV) M813 was originally isolated from the Southeast Asian rodent Mus cervicolor . As with the ecotropic MuLVs derived from Mus musculus , its host range is limited to rodent cells. Earlier studies have mapped its receptor to chromosome 2, but it has not been established whether M813 shares a common receptor with any other MuLVs. In this study, we have performed interference assays with M813 and viruses from four interference groups of MuLV. The infection efficiency of M813 was not compromised in cells expressing any one of the other MuLVs, demonstrating that M813 must use a distinct receptor for cell entry. The entire M813 env coding region was molecularly cloned. Sequence analysis revealed high similarity with other MuLVs but with a unique receptor-binding domain. Substitution of M813 env sequences in Moloney MuLV resulted in a replication-competent virus with a host range and interference profile similar to those of the biological clone M813. M813 thus defines a novel receptor interference group of type C MuLVs. |
| Databáze: | OpenAIRE |
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